Abstract
An expanding choice of ancestry-informative marker single nucleotide polymorphisms (AIM-SNPs) is becoming available for the forensic user in the form of sensitive SNaPshot-based tests or in alternative single-base extension genotyping systems (e.g., Sequenom iPLEX) that can be adapted for analysis with SNaPshot. In addition, alternative ancestry-informative variation: Indels and STRs can be analyzed using direct PCR-to-CE techniques that offer the possibility to detect mixed profiles. We review the current forensically viable AIM panels, their optimized PCR multiplexes, and the population differentiation power they offer. We also describe how improved population divergence balance can be achieved with the enlarged multiplex scales of next-generation sequencing approaches to enable analysis of admixed individuals without biased estimation of co-ancestry proportions.
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1 Electronic Supplementary Material
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Supplementary File S1
Excel file of primer designs, reaction mix ratios, and expected product sizes for forensic AIM panels developed in the authors laboratory: (A) revised 34-plex [7]; (B) Eurasiaplex [8]; (C) 46 AIM-Indels [10]; (D) 12 AIM-STRs [11]. Note primer details are summarized in this format to allow easy copy-paste of sequences (XLSX 30 kb)
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Phillips, C., Santos, C., Fondevila, M., Carracedo, Á., Lareu, M.V. (2016). Inference of Ancestry in Forensic Analysis I: Autosomal Ancestry-Informative Marker Sets. In: Goodwin, W. (eds) Forensic DNA Typing Protocols. Methods in Molecular Biology, vol 1420. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3597-0_18
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DOI: https://doi.org/10.1007/978-1-4939-3597-0_18
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