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Endocannabinoid Signaling pp 9–18Cite as

Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1412))

Abstract

Extraction and quantification of endocannabinoids (eCBs) from biological tissues are essential to unravel their changes in physiological and pathophysiological conditions. We describe here an analytical protocol for extraction of endocannabinoids, anandamide (AEA) and 2-arachidonoyl glycerol (2-AG), endocannabinoid-like lipids such as palmitoyl ethanolamide (PEA) and oleoyl ethanolamide (OEA), as well as arachidonic acid (AA) from biological tissues using liquid-liquid extraction method and simultaneous quantification by liquid chromatography multiple reaction monitoring (LC/MRM).

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References

  1. Zoerner AA, Gutzki FM, Batkai S et al (2011) Quantification of endocannabinoids in biological systems by chromatography and mass spectrometry: a comprehensive review from an analytical and biological perspective. Biochim Biophys Acta 1811:706–723

    Article  CAS  PubMed  Google Scholar 

  2. Buczynski MW, Parsons LH (2010) Quantification of brain endocannabinoid levels: methods, interpretations and pitfalls. Br J Pharmacol 160:423–442

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Lerner R, Lutz B, Bindila L (2013) Tricks and tracks in the identification and qualification of endocannabinoids. eLS. Wiley, Chichester. doi:10.1002/9780470015902.a0023407

    Google Scholar 

  4. Duerr GD, Heinemann JC, Gestrich C et al (2014) Impaired border zone formation and adverse remodeling after reperfused myocardial infarction in cannabinoid CB2 receptor deficient mice. Life Sci 14:324–325

    Google Scholar 

  5. Lomazzo E, Bindila L, Remmers F et al (2015) Therapeutic potential of inhibitors of endocannabinoid degradation for the treatment of stress-related hyperalgesia in an animal model of chronic pain. Neuropsychopharmacology 40:488–501

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Jergas B, Schulte K, Bindila L et al (2014) O-2050 facilitates noradrenaline release and increases the CB1 receptor inverse agonistic effect of rimonabant in the guinea pig hippocampus. Naunyn Schmiedebergs Arch Pharmacol 387:621–628

    Article  CAS  PubMed  Google Scholar 

  7. Wenzel D, Matthey M, Bindila L et al (2013) Endocannabinoid anandamide mediates hypoxic pulmonary vasoconstriction. Proc Natl Acad Sci U S A 110:18710–18715

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgments

This work has been financially supported by Collaborative Research Unit of the German Research Council FOR926 (central project CP1) and BMBF collaborative project LOGIN. We thank Claudia Schwitter, Raissa Lerner, and Julia Post for technical support in establishing the protocols.

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Authors and Affiliations

  1. Institute for Physiological Chemistry, University Medical Center, Duesbergweg 6, 55128, Mainz, Germany

    Laura Bindila & Beat Lutz

Authors
  1. Laura Bindila
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  2. Beat Lutz
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Corresponding author

Correspondence to Beat Lutz .

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Editors and Affiliations

  1. Campus Bio-Medico University of Rome, Department of Medicine, Rome, Italy

    Mauro Maccarrone

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Bindila, L., Lutz, B. (2016). Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues. In: Maccarrone, M. (eds) Endocannabinoid Signaling. Methods in Molecular Biology, vol 1412. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3539-0_2

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  • DOI: https://doi.org/10.1007/978-1-4939-3539-0_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3537-6

  • Online ISBN: 978-1-4939-3539-0

  • eBook Packages: Springer Protocols

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