Abstract
Fatty acid amide hydrolase (FAAH) is an intracellular enzyme responsible for the hydrolysis of endogenous anandamide (AEA), a reaction that terminates the biological effects of this lipid mediator. The final products of this reaction are arachidonic acid and ethanolamine. In the method described herein, FAAH activity is measured through the use of a radioactive substrate by quantification of reaction products, that is, [14C]-ethanolamine from [14C-ethanolamine]-AEA.
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Bari, M., Feole, M., Maccarrone, M. (2016). Assay of FAAH Activity. In: Maccarrone, M. (eds) Endocannabinoid Signaling. Methods in Molecular Biology, vol 1412. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3539-0_14
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DOI: https://doi.org/10.1007/978-1-4939-3539-0_14
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