Abstract
Ever since the discovery of RNA interference (RNAi), therapeutic delivery of siRNA has attracted a lot of interest. However, due to the nature and structure of siRNA, a carrier is needed for any mode of systemic treatment. Furthermore, specific imaging techniques are required to trace where the deposition of the siRNA occurs throughout the body after treatment. Tracking in vivo siRNA biodistribution allows understanding and interpreting therapeutics effects and side effects. A great advantage of noninvasive imaging techniques such as SPECT imaging is that several time points can be assessed in the same subject. Thus, the time course of biodistribution or metabolic processes can be followed. Therefore, we have described an approach to modify siRNA with a DTPA (Diethylene Triamine Pentaacetic Acid) chelator in order to utilize an indium labeled siRNA for SPECT imaging. Here, we explain the details of the labeling and purification procedures.
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Acknowledgements
This work was supported by the NanoIncubator grant to Olivia Merkel as well as the Ruth L. Kirschstein National Research Award T32-CA009531 fellowship to Steven Jones.
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Jones, S., Merkel, O. (2016). Indium-Labeling of siRNA for Small Animal SPECT Imaging. In: Medarova, Z. (eds) RNA Imaging. Methods in Molecular Biology, vol 1372. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3148-4_6
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DOI: https://doi.org/10.1007/978-1-4939-3148-4_6
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3147-7
Online ISBN: 978-1-4939-3148-4
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