Protocol

Yeast Cytokinesis

Volume 1369 of the series Methods in Molecular Biology pp 293-308

Fission Yeast Cell Cycle Synchronization Methods

  • Marta Tormos-PérezAffiliated withInstituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas/Universidad de SalamancaInstituto de Investigación Biomédica de Salamanca (IBSAL)
  • , Livia Pérez-HidalgoAffiliated withInstituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas/Universidad de SalamancaInstituto de Investigación Biomédica de Salamanca (IBSAL)
  • , Sergio MorenoAffiliated withInstituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas/Universidad de SalamancaInstituto de Investigación Biomédica de Salamanca (IBSAL) Email author 

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Abstract

Fission yeast cells can be synchronized by cell cycle arrest and release or by size selection. Cell cycle arrest synchronization is based on the block and release of temperature-sensitive cell cycle mutants or treatment with drugs. The most widely used approaches are cdc10-129 for G1; hydroxyurea (HU) for early S-phase; cdc25-22 for G2, and nda3-KM311 for mitosis. Cells can also be synchronized by size selection using centrifugal elutriation or a lactose gradient. Here we describe the methods most commonly used to synchronize fission yeast cells.

Key words

Fission yeast Schizosaccharomyces pombe Synchronization Block and release cdc10-129 cdc25-22 nda3-KM311 HU Centrifugal elutriation Lactose gradient G1-phase S-phase G2-phase Mitosis