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Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry

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Plant Signal Transduction

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1363))

Abstract

Virus-Induced Gene Silencing (VIGS) is an excellent reverse genetic tool for the study of gene function in plants, based on virus infection. In this chapter, we describe a high-throughput approach based on VIGS for the study of tomato fruit biochemistry. It comprises the selection of the sequence for silencing using bioinformatics tools, the cloning of the fragment in the Tobacco Rattle Virus (TRV), and the agroinfiltration of tomato fruits mediated by Agrobacterium tumefaciens.

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Acknowledgments

This work was supported by the Italian Ministry of Research (Project “Integrated Knowledge for the Sustainability and Innovation of Italian Agri-Food”), by the Italian Ministry of Agriculture (Projects “Nutrisol” and “Biomassval”), and by the European Commission (FP7 Project “From discovery to products: A next generation pipeline for the sustainable generation of high-value plant products,” contract no. 613513; Horizon 2020 project “Traditional tomato varieties and cultural practices: a case for agricultural diversification with impact on food security and health of European population,” contract no. 634561) and benefited from the networking activities of COST Action FA1106 “QualityFruit.” We thank Alessandro Nicolia for critical reading of the manuscript.

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Authors and Affiliations

  1. Trisaia Research Center, ENEA (Italian National Agency for New Technologies, Energy, and Sustainable Development), 75026, Rotondella, MT, Italy

    Elio Fantini

  2. Casaccia Research Center, ENEA (Italian National Agency for New Technologies, Energy, and Sustainable Development), via Anguillarese 301, 00123, Rome, Italy

    Giovanni Giuliano

Authors
  1. Elio Fantini
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  2. Giovanni Giuliano
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Corresponding authors

Correspondence to Elio Fantini or Giovanni Giuliano .

Editor information

Editors and Affiliations

  1. The University of Queensland, St. Lucia, Queensland, Australia

    Jose R. Botella

  2. Universidad de Málaga, Málaga, Spain

    Miguel A. Botella

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Fantini, E., Giuliano, G. (2016). Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry. In: Botella, J., Botella, M. (eds) Plant Signal Transduction. Methods in Molecular Biology, vol 1363. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3115-6_7

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  • DOI: https://doi.org/10.1007/978-1-4939-3115-6_7

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3114-9

  • Online ISBN: 978-1-4939-3115-6

  • eBook Packages: Springer Protocols

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