Abstract
Transcription start sites are commonly used to locate promoter elements in bacterial genomes. TSS were previously studied one gene at a time, often through 5′-rapid amplification of cDNA ends (5′-RACE). This technique has now been adapted for high-throughput sequencing and can be used to precisely identify TSS in a genome-wide fashion for practically any bacterium, which greatly contributes to our understanding of gene regulatory networks in microorganisms.
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Acknowledgment
We thank Pierre-Étienne Jacques for technical assistance, Thomas Knight Jr. for M. florum genome annotations, the Centre de calcul scientifique of Université de Sherbrooke for computational resources and technical support, and Vincent Baby for its precious comments on the manuscript. This work was supported by the Fonds québécois de la recherche sur la nature et les technologies through a MSc scholarship awarded to D.M. and a Projet de recherche en équipe grant awarded to S.R. and Vincent Burrus. S.R. holds a Chercheur-Boursier Junior 1 award from the Fonds de recherche Québec-Santé.
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Matteau, D., Rodrigue, S. (2015). Precise Identification of Genome-Wide Transcription Start Sites in Bacteria by 5′-Rapid Amplification of cDNA Ends (5′-RACE). In: Leblanc, B., Rodrigue, S. (eds) DNA-Protein Interactions. Methods in Molecular Biology, vol 1334. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2877-4_9
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DOI: https://doi.org/10.1007/978-1-4939-2877-4_9
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