Abstract
Deep sequencing has many possible applications; one of them is the identification and quantification of RNA editing sites. The most common type of RNA editing is adenosine to inosine (A-to-I) editing. A prerequisite for this editing process is a double-stranded RNA (dsRNA) structure. Such dsRNAs are formed as part of the microRNA (miRNA) maturation process, and it is therefore expected that miRNAs are affected by A-to-I editing. Indeed, tens of editing sites were found in miRNAs, some of which change the miRNA binding specificity. Here, we describe a protocol for the identification of RNA editing sites in mature miRNAs using deep sequencing data.
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Acknowledgments
This work was supported by a grant from the Israel Science Foundation [379/12] and a grant from the United States–Israel Binational Science Foundation (grant number 2009/290), Jerusalem, Israel, to E.E. S.A. was supported by a Clore Fellowship.
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Alon, S., Eisenberg, E. (2015). Using Deep Sequencing Data for Identification of Editing Sites in Mature miRNAs. In: Picardi, E. (eds) RNA Bioinformatics. Methods in Molecular Biology, vol 1269. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2291-8_14
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DOI: https://doi.org/10.1007/978-1-4939-2291-8_14
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