Protocol

Cardiac Tissue Engineering

Volume 1181 of the series Methods in Molecular Biology pp 167-176

Date:

Collagen-Based Engineered Heart Muscle

  • Malte TiburcyAffiliated withInstitute of Pharmacology, Heart Research Center Göttingen (HRCG), University Medical Center GöttingenDZHK (German Center for Cardiovascular Research), partner site Göttingen
  • , Tim MeyerAffiliated withInstitute of Pharmacology, Heart Research Center Göttingen (HRCG), University Medical Center GöttingenDZHK (German Center for Cardiovascular Research), partner site Göttingen
  • , Poh Loong SoongAffiliated withInstitute of Pharmacology, Heart Research Center Göttingen (HRCG), University Medical Center GöttingenDZHK (German Center for Cardiovascular Research), partner site Göttingen
  • , Wolfram-Hubertus ZimmermannAffiliated withInstitute of Pharmacology, Heart Research Center Göttingen (HRCG), University Medical Center GöttingenDZHK (German Center for Cardiovascular Research), partner site Göttingen Email author 

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Abstract

Cardiac muscle engineering has evolved over nearly 20 years from a scientific oddity to a mainstream technology with a wide range of applications. Of the many published methods it appears that hydrogels constitute the preferred scaffolds for myocardial tissue engineering and support of organotypic development. Here we describe a simple and highly robust protocol for the generation of engineered heart muscle using a collagen-based hydrogel method.

Key words

Tissue engineering Heart Stem cells