Protocol

Microbial Biofilms

Volume 1147 of the series Methods in Molecular Biology pp 187-201

Date:

In Vitro Screening of Antifungal Compounds Able to Counteract Biofilm Development

  • Marion GirardotAffiliated withLaboratory of ecology and biology of the interactions, Faculty of Medicine Pharmacy, University of Poitiers, UMR CNRS 7267
  • , Christine ImbertAffiliated withLaboratory of ecology and biology of the interactions, Faculty of Medicine Pharmacy, University of Poitiers, UMR CNRS 7267 Email author 

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Abstract

Fungi are able to grow as a single-species or a more complex biofilm attached to inert surfaces (catheters…) or tissues (lung…). This last form is a microbial niche which must be considered as a major risk factor of developing a human fungal infection. Nowadays, only a few therapeutic agents have been shown to be active against fungal biofilms in vitro and/or in vivo. So there is a real need to find new anti-biofilm molecules. Here we describe in detail some rapid, 96-well microtiter plate-based methods, for the screening of compounds with anti-biofilm activity against Candida spp. yeasts. Two approaches will be considered: prophylactic or curative effects of the tested compounds by producing biofilms on two supports – polystyrene well surfaces and catheter sections.

Key words

Biofilms Candida spp. Antifungal compounds Catheters Microtiter plates Fungal infections Colorimetric assay XTT