Protocol

Crop Breeding

Volume 1145 of the series Methods in Molecular Biology pp 203-211

Date:

Agrobacterium-Mediated Transformation of Barley (Hordeum vulgare L.)

  • Ainur IsmagulAffiliated withAustralian Centre for Plant Functional Genomics, University of AdelaideSchool of Agriculture, Food and Wine, Waite Research Institute, University of Adelaide
  • , Iryna MazonkaAffiliated withAustralian Centre for Plant Functional Genomics, University of AdelaideSchool of Agriculture, Food and Wine, Waite Research Institute, University of Adelaide
  • , Corinne CallegariAffiliated withAustralian Centre for Plant Functional Genomics, University of AdelaideSchool of Agriculture, Food and Wine, Waite Research Institute, University of Adelaide
  • , Serik ElibyAffiliated withAustralian Centre for Plant Functional Genomics, University of AdelaideSchool of Agriculture, Food and Wine, Waite Research Institute, University of Adelaide Email author 

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Barley biotechnology requires efficient genetic engineering tools for producing transgenic plants necessary for conducting reverse genetics analyses in breeding and functional genomics research. Agrobacterium-mediated genetic transformation is an important technique for producing barley transgenics with simple low-copy number transgenes. This chapter reports a refined protocol for the systematic high-throughput transformation of the advanced Australian spring barley breeding line WI4330.

Key words

Agrobacterium Barley Biotechnology Cereals Transgenic