Protocol

Crop Breeding

Volume 1145 of the series Methods in Molecular Biology pp 191-202

Date:

Biolistic Transformation of Wheat with Centrophenoxine as a Synthetic Auxin

  • Ainur IsmagulAffiliated withAustralian Centre for Plant Functional Genomics, University of AdelaideSchool of Agriculture, Food and Wine, Waite Research Institute, University of Adelaide
  • , Gulnur IskakovaAffiliated withAustralian Centre for Plant Functional Genomics, University of AdelaideSchool of Agriculture, Food and Wine, Waite Research Institute, University of Adelaide
  • , John C. HarrisAffiliated withAustralian Centre for Plant Functional Genomics, University of AdelaideSchool of Agriculture, Food and Wine, Waite Research Institute, University of Adelaide
  • , Serik ElibyAffiliated withAustralian Centre for Plant Functional Genomics, University of AdelaideSchool of Agriculture, Food and Wine, Waite Research Institute, University of Adelaide Email author 

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Abstract

Cereal crops, including bread wheat (Triticum aestivum L.), are an important staple food worldwide. With a growing global population, it is evident that current crop production will not meet the rising demands being placed on modern agriculture. Efforts to improve crop yield and stress-tolerance by traditional breeding are labor intensive, time consuming, and highly dependent upon the ability to capture existing and novel genetic variation from a restricted genetic pool. Genetic engineering of crop species is one of several alternatives to traditional breeding for the introduction of novel genetic variation. This recently established technology has proved useful for the introduction of novel traits like pest resistance and herbicide tolerance. As a universal tool for genetic transformation, the Biolistic Gene Gun allows for the genomic integration of novel gene sequences from various sources into a whole host of living organisms.

In this chapter, we present a novel and detailed protocol for the Biolistic Transformation of bread wheat that uses the pharmaceutical compound, Centrophenoxine (CPX). The application of CPX as the main auxin-like plant growth regulator in cereal genetic transformation replaces the potent but more toxic herbicide 2,4-D.

Key words

Biolistic Cereals Centrophenoxine Microprojectile bombardment Transgenic Wheat