Protocol

Herpes Simplex Virus

Volume 1144 of the series Methods in Molecular Biology pp 19-29

Date:

Herpes Simplex Virus Growth, Preparation, and Assay

  • Peggy MarconiAffiliated withSection of Applied Microbiology and Pathology, Department of Life Sciences and Biotechnology (SVeB), University of Ferrara Email author 
  • , Roberto ManservigiAffiliated withSection of Applied Microbiology and Pathology, Department of Life Sciences and Biotechnology (SVeB), University of Ferrara

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Abstract

In order to study the biology of herpes simplex virus or to use it as a vector in gene therapy, it is necessary to grow the virus and to prepare virus stocks. Many different protocols are available from different research groups working with herpes simplex virus type 1 or 2 (HSV-1 or HSV-2). This chapter describes the procedures used in our laboratory.

Key words

Virus growth Plaque purification Plaque titration Growth curve Virus stock Gradient purification