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Cerebral Angiogenesis

Volume 1135 of the series Methods in Molecular Biology pp 289-305

Novel Methods for Accurate Identification, Isolation, and Genomic Analysis of Symptomatic Microenvironments in Atherosclerotic Arteries

  • Mark SlevinAffiliated withSchool of Healthcare Science, Manchester Metropolitan University
  • , Maribel BaldellouAffiliated withInstitut Català de Ciències Cardiovasculars, Hospital de la Santa Creu i Sant Pau
  • , Elspeth HillAffiliated withDepartment of Vascular Surgery, Central Manchester University Hospitals NHS Foundation Trust
  • , Yvonne AlexanderAffiliated withSchool of Healthcare Science, Manchester Metropolitan University
  • , Garry McDowellAffiliated withUniversity of Edge hill
  • , Christopher MurgatroydAffiliated withSchool of Healthcare Science, Manchester Metropolitan University
  • , Michael CarrollAffiliated withSchool of Healthcare Science, Manchester Metropolitan University
  • , Hans DegensAffiliated withSchool of Healthcare Science, Manchester Metropolitan University
  • , Jerzy KrupinskiAffiliated withDivision of Cerebrovascular Diseases, Department of Neurology, Hospital Universitari
    • , Norma RoviraAffiliated withDivision of Cerebrovascular Diseases, Department of Neurology, Hospital Universitari
    • , Mohammad ChowdhuryAffiliated withCardiovascular Research Group, School of Medicine, University of Manchester
    • , Ferdinand Serracino-InglottAffiliated withCardiovascular Research Group, School of Medicine, University of Manchester
    • , Lina BadimonAffiliated withInstitut Català de Ciències Cardiovasculars, Hospital de la Santa Creu i Sant Pau

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Abstract

A challenge facing surgeons is identification and selection of patients for carotid endarterectomy or coronary artery bypass/surgical intervention. While some patients with atherosclerosis develop unstable plaques liable to undergo thrombosis, others form more stable plaques and are asymptomatic. Identification of the cellular signaling mechanisms associated with production of the inflammatory, hemorrhagic lesions of mature heterogenic plaques will help significantly in our understanding of the differences in microenvironment associated with development of regions susceptible to rupture and thrombosis and may help to predict the risk of plaque rupture and guide surgical intervention to patients who will most benefit. Here, we demonstrate detailed and novel methodologies for successful and, more importantly, accurate and reproducible extraction, sampling, and analysis of micro-regions in stable and unstable coronary/carotid arteries. This information can be applied to samples from other origins and so should be useful for scientists working with micro-isolation techniques in all fields of biomedical science.

Key words

Laser capture Atherosclerotic plaques Microfluidity cards PCR