Abstract
Generation of cardiomyocytes from pluripotent stem cells (PSCs) is a common and valuable approach to produce large amount of cells for various applications, including assays and models for drug development, cell-based therapies, and tissue engineering. All these applications would benefit from a reliable bioreactor-based methodology to consistently generate homogenous PSC-derived embryoid bodies (EBs) at a large scale, which can further undergo cardiomyogenic differentiation. The goal of this chapter is to describe a scalable method to consistently generate large amount of homogeneous and synchronized EBs from PSCs. This method utilizes a slow-turning lateral vessel bioreactor to direct the EB formation and their subsequent cardiomyogenic lineage differentiation.
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Acknowledgements
The research that supports these methodologies was funded by grants from the EU FP7 (“PartnErS” PIAP-GA-2008-218205; “AniStem,” PIAP-GA-2011286264; “EpiHealth,” HEALTH-2012-F2-278418; “EpiHealthNet,” PITN-GA-2012-317146, “STEMMAD,” PIAPP-GA-2012-324451) Research Center of Excellence 9878/2015/FEKUT project, the Mahidol University, the Thailand Research Fund (TRF), the Office of Higher Education Commission, Thailand (OHEC), and the Mahidol University (MRG 5680108).
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Rungarunlert, S., Ferreira, J.N., Dinnyes, A. (2016). Novel Bioreactor Platform for Scalable Cardiomyogenic Differentiation from Pluripotent Stem Cell-Derived Embryoid Bodies. In: Turksen, K. (eds) Bioreactors in Stem Cell Biology. Methods in Molecular Biology, vol 1502. Humana Press, New York, NY. https://doi.org/10.1007/7651_2016_341
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DOI: https://doi.org/10.1007/7651_2016_341
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