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Stimulating Peripheral Afferents to Evoke Cardiorespiratory Reflex Responses in the In Situ Arterially Perfused Preparation

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Stimulation and Inhibition of Neurons

Part of the book series: Neuromethods ((NM,volume 78))

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Abstract

The in situ arterially perfused rodent preparation is an innovation that has allowed for significant progress in the study of cardiorespiratory reflex circuitry. This preparation provides a number of advantages over other preparations. The retention of peripheral cardiorespiratory afferents enables the study of reflex circuitry that is not possible in in vitro slice preparations. In addition, the in situ arterially perfused preparation provides unsurpassed mechanical stability of the brainstem compared with the in vivo preparation. This stability allows for better cellular recordings for prolonged periods.

Here, the basic technique for the in situ arterially perfused preparation including recording of a number of cardiovascular and respiratory parameters is described. In addition, some of the common techniques for stimulating peripheral afferent nerves that produce different cardiovascular and respiratory reflex responses are discussed.

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References

  1. Smith JC, Ellenberger HH, Ballanyi K, Richter DW, Feldman JL (1991) Pre-Botzinger complex: a brainstem region that may generate respiratory rhythm in mammals. Science 254:726–729

    Article  PubMed  CAS  Google Scholar 

  2. Wang X, Huang ZG, Dergacheva O, Bouairi E, Gorini C, Stephens C, Andresen MC, Mendelowitz D (2005) Ketamine inhibits inspiratory-evoked gamma-aminobutyric acid and glycine neurotransmission to cardiac vagal neurons in the nucleus ambiguus. Anesthesiology 103:353–359

    Article  PubMed  CAS  Google Scholar 

  3. Del Negro CA, Morgado-Valle C, Hayes JA, Mackay DD, Pace RW, Crowder EA, Feldman JL (2005) Sodium and calcium current-mediated pacemaker neurons and respiratory rhythm generation. J Neurosci 25:446–453

    Article  PubMed  Google Scholar 

  4. Kline DD, King TL, Austgen JR, Heesch CM, Hasser EM (2010) Sensory afferent and hypoxia-mediated activation of nucleus tractus solitarius neurons that project to the rostral ventrolateral medulla. Neuroscience 167:510–527

    Article  PubMed  CAS  Google Scholar 

  5. McDougall SJ, Peters JH, LaBrant L, Wang X, Koop DR, Andresen MC (2008) Paired assessment of volatile anesthetic concentrations with synaptic actions recorded in vitro. PLoS One 3:e3372

    Article  PubMed  Google Scholar 

  6. Chen Q-H, Andrade MA, Calderon AS, Toney GM (2010) Hypertension induced by angiotensin II and a high salt diet involves reduced SK current and increased excitability of RVLM projecting PVN neurons. J Neurophysiol 104:2329–2337

    Article  PubMed  CAS  Google Scholar 

  7. Bailey TW, Appleyard SM, Jin Y-H, Andresen MC (2008) Organization and Properties of GABAergic Neurons in Solitary Tract Nucleus (NTS). J Neurophysiol 99:1712–1722

    Article  PubMed  Google Scholar 

  8. Ballanyi K, Onimaru H, Homma I (1999) Respiratory network function in the isolated brainstem-spinal cord of newborn rats. Prog Neurobiol 59:583–634

    Article  PubMed  CAS  Google Scholar 

  9. Kron M, Reuter J, Gerhardt E, Manzke T, Zhang W, Dutschmann M (2008) Emergence of brain-derived neurotrophic factor-induced postsynaptic potentiation of NMDA currents during the postnatal maturation of the Kolliker-Fuse nucleus of rat. J Physiol 586:2331–2343

    Article  PubMed  CAS  Google Scholar 

  10. Paton JF (1996) A working heart-brainstem preparation of the mouse. J Neurosci Methods 65:63–68

    Article  PubMed  CAS  Google Scholar 

  11. Pickering AE, Paton JF (2006) A decerebrate, artificially-perfused in situ preparation of rat: utility for the study of autonomic and nociceptive processing. J Neurosci Methods 155:260–271

    Article  PubMed  Google Scholar 

  12. Potts JT, Spyer KM, Paton JF (2000) Somatosympathetic reflex in a working heart-brainstem preparation of the rat. Brain Res Bull 53:59–67

    Article  PubMed  CAS  Google Scholar 

  13. Paton JF, Li YW, Kasparov S (1999) Reflex response and convergence of pharyngoesophageal and peripheral chemoreceptors in the nucleus of the solitary tract. Neuroscience 93:143–154

    Article  PubMed  CAS  Google Scholar 

  14. Smith JE, Paton JF, Andrews PL (2002) An arterially perfused decerebrate preparation of Suncus murinus (house musk shrew) for the study of emesis and swallowing. Exp Physiol 87:563–574

    Article  PubMed  CAS  Google Scholar 

  15. Smith JE, Paton JF, Andrews PL (2001) Cardiorespiratory reflexes in a working ­heart-brainstem preparation of the house musk shrew, Suncus murinus. Auton Neurosci 89:54–59

    Article  PubMed  CAS  Google Scholar 

  16. Gajda BM, Fong AY, Milsom WK (2010) Species differences in respiratory rhythm ­generation in rodents. Am J Physiol Regul Integr Comp Physiol 298:R887–R898

    Article  PubMed  CAS  Google Scholar 

  17. Dutschmann M, Wilson RJ, Paton JF (2000) Respiratory activity in neonatal rats. Auton Neurosci 84:19–29

    Article  PubMed  CAS  Google Scholar 

  18. Fong AY, Potts JT (2008) Neurokinin-1 receptors modulate the excitability of expiratory neurons in the ventral respiratory group. J Neurophysiol 99:900–914

    Article  PubMed  CAS  Google Scholar 

  19. Potts JT, Rybak IA, Paton JF (2005) Respiratory rhythm entrainment by somatic afferent stimulation. J Neurosci 25:1965–1978

    Article  PubMed  CAS  Google Scholar 

  20. Fong AY, Potts JT (2006) Neurokinin-1 receptor activation in Botzinger complex evokes bradypnoea. J Physiol 575:869–885

    Article  PubMed  CAS  Google Scholar 

  21. Potts JT, Fong AY, Anguelov PI, Lee S, McGovern D, Grias I (2007) Targeted deletion of neurokinin-1 receptor expressing nucleus tractus solitarii neurons precludes somatosensory depression of arterial baroreceptor-heart rate reflex. Neuroscience 145:1168–1181

    Article  PubMed  CAS  Google Scholar 

  22. Boscan P, Paton JF (2002) Integration of cornea and cardiorespiratory afferents in the nucleus of the solitary tract of the rat. Am J Physiol Heart Circ Physiol 282:H1278–H1287

    PubMed  CAS  Google Scholar 

  23. Boscan P, Paton JF (2001) Role of the solitary tract nucleus in mediating nociceptive evoked cardiorespiratory responses. Auton Neurosci 86:170–182

    Article  PubMed  CAS  Google Scholar 

  24. Harris MB, St-John WM (2005) Phasic pulmonary stretch receptor feedback modulates both eupnea and gasping in an in situ rat preparation. Am J Physiol Regul Integr Comp Physiol 289:R450–R455

    Article  PubMed  CAS  Google Scholar 

  25. Molkov YI, Zoccal DB, Moraes DJA, Paton JFR, Machado BH, Rybak IA (2011) Intermittent hypoxia-induced sensitization of central chemoreceptors contributes to sympathetic nerve activity during late expiration in rats. J Neurophysiol 105:3080–3091

    Article  PubMed  Google Scholar 

  26. Abdala AP, Rybak IA, Smith JC, Paton JF (2009) Abdominal expiratory activity in the rat brainstem-spinal cord in situ: patterns, origins and implications for respiratory rhythm generation. J Physiol 587:3539–3559

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Australian School of Advanced Medicine, Macquarie University, North Ryde, NSW, Australia

    Angelina Y. Fong

Authors
  1. Angelina Y. Fong
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Correspondence to Angelina Y. Fong .

Editor information

Editors and Affiliations

  1. , Australian School of Advanced Medicine, Macquarie University, Technology Place 2, North Ryde, 2113, New South Wales, Australia

    Paul M. Pilowsky

  2. , Australian School of Advanced Medicine, Macquarie University, Technology Place 2, North Ryde, 2113, New South Wales, Australia

    Melissa M.J. Farnham

  3. , Australian School of Advanced Medicine, Macquarie University, Technology Place 2, North Ryde, 2113, New South Wales, Australia

    Angelina Y. Fong

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Fong, A.Y. (2013). Stimulating Peripheral Afferents to Evoke Cardiorespiratory Reflex Responses in the In Situ Arterially Perfused Preparation. In: Pilowsky, P., Farnham, M., Fong, A. (eds) Stimulation and Inhibition of Neurons. Neuromethods, vol 78. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-233-9_2

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  • DOI: https://doi.org/10.1007/978-1-62703-233-9_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-232-2

  • Online ISBN: 978-1-62703-233-9

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