Abstract
Lipidic magnetic nanoparticles are a rapid and reliable magnet-guided nucleic acid delivery system, which we have named as “LipoMag” (Namiki et al., Nat. Nanotechnol 4:598–606, 2009). LipoMag is composed of a cationic lipid shell, an oleic acid binder, and an iron oxide nanocrystal core. Through the electrostatic force, positively charged LipoMag and negatively charged nucleic acid form complexes, termed “mag-lipoplex.” Under a magnetic field, LipoMag bearing nucleic acid can be controllably accumulated in target cells. Here we describe the procedure for the preparation of LipoMag which can be used for the transfection of nucleic acids, such as plasmid DNA and small interfering RNA.
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Acknowledgment
I We dedicate this work to the late T. Terada and the late K. Nariai. I We would like to acknowledge Yukiko Ishii, Yoko Yumoto, Yuki Nagase, and Shuichi Nakagawa for their assistance. This work was supported by a Funding Program for Next Generation World-Leading Researchers (LS114) from JSPS, an Industrial Technology Research Grant Program (08C46049a) from the NEDO of Japan, Grant-in-Aid for Scientific Research (#22300180) from JSPS, and the Iketani Science and Technology Foundation (2012).
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Namiki, Y. (2013). Synthesis of Lipidic Magnetic Nanoparticles for Nucleic Acid Delivery. In: Ogris, M., Oupicky, D. (eds) Nanotechnology for Nucleic Acid Delivery. Methods in Molecular Biology, vol 948. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-140-0_16
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DOI: https://doi.org/10.1007/978-1-62703-140-0_16
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