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Nanoimaging pp 293–311Cite as

Preparation of Gold Nanocluster Bioconjugates for Electron Microscopy

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 950))

Abstract

In this chapter, we describe types of gold nanoparticle–biomolecule conjugates and their use in electron microscopy. Included are two detailed protocols for labeling an IgG antibody with gold monolayer protected clusters. The first approach is a direct bonding approach that utilizes the ligand place exchange reaction. The second approach describes NHS–EDC coupling of Au144(pMBA)60 with IgG. Also included are various characterization techniques for determining labeling efficiency.

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Authors and Affiliations

  1. Department of Chemistry, Colorado State University, Fort Collins, CO, USA

    Christine L. Heinecke & Christopher J. Ackerson

Authors
  1. Christine L. Heinecke
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  2. Christopher J. Ackerson
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Corresponding author

Correspondence to Christopher J. Ackerson .

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Editors and Affiliations

  1. Nat. Inst. Biomedical Imaging & Bioengin, National Institutes of Health, South Drive 13, Bethesda, 20892, Maryland, USA

    Alioscka A. Sousa

  2. , National Cancer Institute, National Institutes of Health, Center Drive 10, Bethesda, 20892, USA

    Michael J. Kruhlak

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Heinecke, C.L., Ackerson, C.J. (2013). Preparation of Gold Nanocluster Bioconjugates for Electron Microscopy. In: Sousa, A., Kruhlak, M. (eds) Nanoimaging. Methods in Molecular Biology, vol 950. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-137-0_17

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  • DOI: https://doi.org/10.1007/978-1-62703-137-0_17

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-136-3

  • Online ISBN: 978-1-62703-137-0

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