Abstract
Inositol(1,4,5)trisphosphate [Ins(1,4,5)P3] is an important second messenger that activates its cognate Ins(1,4,5)P3 receptor to release Ca2+ from intracellular stores. The assay described in this chapter uses the Ins(1,4,5)P3 receptor (essentially as a binding protein) to measure the biologically active trisphosphate (specifically from other trisphosphates). The binding protein (Ins(1,4,5)P3 receptor) is prepared from Bovine adrenal glands and this is mixed with [3H]-labeled and unlabeled (generated from biological samples or standards) Ins(1,4,5)P3. Using the same principles as for radioimmunoassay/ELISA the mass of Ins(1,4,5)P3 in biological samples can be estimated.
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Smart, D. (2013). Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay. In: Lambert, D., Rainbow, R. (eds) Calcium Signaling Protocols. Methods in Molecular Biology, vol 937. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-086-1_11
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DOI: https://doi.org/10.1007/978-1-62703-086-1_11
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-086-1
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