Abstract
Hydrangea (Hortensia) is a highly popular ornamental plant for garden decoration, and now it is commercially produced for cut flower branches. For in vitro culture, Murashige and Skoog medium supplemented with BA (0.25 mg/L) and sucrose (30 g/L) was used. Culture conditions were 23 ± 1°C of temperature, light intensity of 35 μmol/m2/s P.P.F.D., and 16/8 h day/night photoperiod. Following shoot proliferation, the in vitro rooting frequency was 100% on a medium containing NAA 0.5 mg/L. However, 95% direct in vivo rooting was achieved by dipping microcuttings in a 5,000 ppm K-IBA solution which were transferred afterward to a glasshouse for acclimatization. After 21 days, fully acclimatized and well-established plants were obtained, suitable for commercialization. Furthermore, leaf fragments derived from in vitro plantlets were cultured for callus induction and adventitious shoot regeneration.
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References
Bailey LH, Bailey EZ (1976) Hortus third. MacMillan, New York, p 275
Dirr MA, Heuser CW Jr (1987) The reference manual of woody plant propagation: from seed to tissue culture. Varsity Press, Athens
Young J, Young C (1992) Seed of woody plants in North America, revised and enlarged edn. Dioscorides Press, Portland
Hill L, Hill N (1995) Hydrangea. Country J July/Aug 1995:70–71
Hartmann HT, Kester DE, Davies FT Jr, Geneve RL (1997) Plant propagation: principles and practices, 6th edn. Prentice-Hall, Upper Saddle River
Jacobs RM, Berry J, Duck P (1990) New propagation techniques. Comb Proc Int Plant Prop Soc 40:394–396
Sebastian TK, Heurser CW (1987) In vitro propagation of Hydrangea quercifolia Bartr. Sci Hortic 31:303–309
Ledbetter DI, Preece JE (2004) Thidiazuron stimulates adventitious shoot production from Hydrangea quercifolia Bartr. leaf explants. Sci Hortic 101:121–126
Sacco E, Savona M, Antonetti M, Grassotti A, Pasqualetto PL, Ruffoni B (2010) In vitro propagation and regeneration of several Hydrangea genotypes. Acta Hortic (in press)
Abou Dahab TAM (2007) In vitro propagation of Hydrangea macrophylla Thunb. Arab J Biotechnol 10:161–178
Doil A, Zhang R, Schum A, Serek M, Wilkelmann T (2008) In vitro regeneration and propagation of Hydrangea macrophylla Thunb. “Nachtigall”. Propag Ornam Plants 8:151–153
Boccon-Gibod J, Billard C, Maltete S (2000) In vitro regeneration system of Hydrangea macrophylla plantlets from leaves and internodes. Acta Hortic 508:229–231
Liu F, Li-Huang L, Li YL, Reinhoud P, Jongsma MA, Wang CY (2011) Shoot organogenesis in leaf explants of Hydrangea macrophylla Hyd1 and assessing genetic stability of regenerants using ISSR markers. Plant Cell Tissue Organ Cult 104:111–117
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirement suspension cultures of soybean root cells. Exp Cell Res 50:151–158
Acknowledgements
The results reported in this paper comes from the project “Valorizzazione a scopi commerciali del genere Hydrangea” supported by the Italian Ministry of Agriculture (D.M. I1063/7643/09) in collaboration with the CRA-VIV of Pescia, Italy, and the Company “Meristema” di Pasqualetto P.L. (Cascine di Buti, Italy).
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Ruffoni, B., Sacco, E., Savona, M. (2012). In Vitro Propagation of Hydrangea spp.. In: Lambardi, M., Ozudogru, E., Jain, S. (eds) Protocols for Micropropagation of Selected Economically-Important Horticultural Plants. Methods in Molecular Biology, vol 994. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-074-8_18
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DOI: https://doi.org/10.1007/978-1-62703-074-8_18
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