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Amyloid Proteins pp 493–506Cite as

Tissue Processing Prior to Analysis of Alzheimer’s Disease Associated Proteins and Metabolites, Including Aβ

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 849))

Abstract

Amyloid-containing tissue, whether from human patients or an animal model of a disease, is typically characterized by various biochemical and immunohistochemical techniques, many of which are described in detail in this volume. In this chapter, we describe a straightforward technique for the homogenization of tissue prior to these analyses. The technique is particularly well suited for performing a large number of different biochemical analyses on a single mouse brain hemisphere. Starting with this homogenate multiple characterizations can be done, including western blot analysis and isolation of membrane-associated proteins, both of which are described here. Additional analyses can readily be performed on the tissue homogenate, including the ELISA quantitation of Aβ in the brain of a transgenic mouse model of β-amyloid deposition. The ELISA technique is described in detail in Chapter 34.

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Acknowledgments

This work has been supported by the Alzheimer’s Association (IIRG-07-60047 to P.M.M.) and the National Institute on Aging (AG017617 to P.M.M., R.A.N.). We thank Dr. Karen Duff (Columbia University) for the transgenic mouse brains used in the sample data.

Author information

Authors and Affiliations

  1. Center for Dementia Research, Nathan S. Kline Institute for Psychiatric Research, Orangeburg, NY, USA

    Stephen D. Schmidt, Ralph A. Nixon & Paul M. Mathews

  2. Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA

    Stephen D. Schmidt

  3. Departments of Psychiatry and Cell Biology, New York University School of Medicine, New York, NY, USA

    Ralph A. Nixon

  4. Department of Psychiatry, New York University School of Medicine, New York, NY, USA

    Paul M. Mathews

Authors
  1. Stephen D. Schmidt
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  2. Ralph A. Nixon
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  3. Paul M. Mathews
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Corresponding author

Correspondence to Stephen D. Schmidt .

Editor information

Editors and Affiliations

  1. School of Medicine, Dept. Psychiatry, New York University, First Ave. 550, New York, 10016, New York, USA

    Einar M. Sigurdsson

  2. Centro Nacional de Microbiología, Instituto de Salud Carlos III, Carretera Majadahonda-Pozuelo Km 2, Madrid, 28220, Spain

    Miguel Calero

  3. Inst. Química Física, Rocasolano, CSIC Madrid, C/ Serrano 119, Madrid, 28006, Spain

    María Gasset

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Schmidt, S.D., Nixon, R.A., Mathews, P.M. (2012). Tissue Processing Prior to Analysis of Alzheimer’s Disease Associated Proteins and Metabolites, Including Aβ. In: Sigurdsson, E., Calero, M., Gasset, M. (eds) Amyloid Proteins. Methods in Molecular Biology, vol 849. Humana Press. https://doi.org/10.1007/978-1-61779-551-0_33

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  • DOI: https://doi.org/10.1007/978-1-61779-551-0_33

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-550-3

  • Online ISBN: 978-1-61779-551-0

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