Abstract
The myriad downstream applications of ancient DNA (aDNA) analysis all ultimately require that sequence data are generated from extracts of ancient material. DNA extraction from tissues known to contain preserved biomolecules (e.g. teeth, hair, tissue, bone) relies on subtle modifications of a basic technique that has been in use for nearly two decades. Multiple DNA extraction protocols have been introduced, with varying levels of success depending on tissue type and the long-term preservation environment to which the ancient tissue was exposed. Here, we describe the phenol–chloroform method for extracting aDNA from any tissue type. This commonly employed method allows for the recovery of total nucleic acid content with minimal loss of low molecular weight double-stranded DNA.
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References
Hagelberg E, Clegg JB (1991) Isolation and characterization of DNA from archaeological bone. Proc Biol Sci 244:45–50
Hagelberg E, Sykes B, Hedges R (1989) Ancient bone DNA amplified. Nature 342:485
Hofreiter M, Serre D, Poinar HN, Kuch M, Pääbo S (2001) Ancient DNA. Nat Rev Genet 2:353–359
Cooper A, Poinar HN (2000) Ancient DNA: do it right or not at all. Science 289:1139
Leonard JA, Shanks O, Hofreiter M, Kreuz E, Hodges L, Ream W, Wayne RK, Fleischer RC (2007) Animal DNA in PCR reagents plagues ancient DNA research. J Archaeol Sci 34:1361–1366
Rohland N, Hofreiter M (2007) Ancient DNA extraction from bones and teeth. Nat Protoc 2:1756–1762
Handt O, Höss M, Krings M, Pääbo S (1994) Ancient DNA: methodological challenges. Experientia 50:524–527
Rohland N, Hofreiter M (2007) Comparison and optimization of ancient DNA extraction. Biotechniques 42:343–352
Poinar HN (2002) The genetic secrets some fossils hold. Acc Chem Res 35:676–684
Vasan S, Zhang X, Kapurnitou A, Bernhagen J, Teichberg S, Basgen J, Wagle D, Shih D, Terlecky I, Bucala R, Cerami A, Egan J, Uhlrich P (1996) An agent cleaving glucose-derived protein crosslinks in vitro and in vivo. Nature 382:275–278
Adler CJ, Haak W, Donlon D, Cooper A (2010) Survival and recovery of DNA from ancient teeth and bones. J Archaeol Sci 38(5):956–964
Gilbert MTP, Wilson AS, Bunce M, Hansen AJ, Willerslev E, Shapiro B, Higham TFG, Richards MP, O’Connell TC, Tobin DJ, Janaway RC, Cooper A (2004) Ancient mitochondrial DNA from hair. Curr Biol 14:R463–R464
Lindahl T (1993) Instability and decay of the primary structure of DNA. Nature 362:709–715
Acknowledgments
Thanks to Beth Shapiro for the opportunity to contribute this chapter.
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Barnett, R., Larson, G. (2012). A Phenol–Chloroform Protocol for Extracting DNA from Ancient Samples. In: Shapiro, B., Hofreiter, M. (eds) Ancient DNA. Methods in Molecular Biology, vol 840. Humana Press. https://doi.org/10.1007/978-1-61779-516-9_2
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DOI: https://doi.org/10.1007/978-1-61779-516-9_2
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Online ISBN: 978-1-61779-516-9
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