Abstract
Alcoholism is a chronic treatment-resistant disorder typically presenting with recurrent/cyclic periods of abusive drinking, withdrawal, abstinence, and relapse. Various strategies that attempt to model these processes in animals have been developed to elucidate the behavioral and neural processes underlying alcoholism. Many of these have involved chronic ethanol exposure and withdrawal with the most widely employed methods involving mice or rats. Prominent features of these methods include alcohol vapor or intragastric forced exposure, cyclic or intermittent periods of alcohol availability with various lengths of forced abstinence, voluntary consumption, the use of genetically alcohol-preferring animals, and inclusion of various pharmacological or environmental challenges to worsen or mitigate symptoms. This chapter emphasizes alcohol exposure and withdrawal and discusses representative metrics used to monitor the consequences of employing these methods. These include but are not limited to intensity and pattern of alcohol exposure, seizure sensitivity during withdrawal, and emotional responding.
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References
Ballenger, J. C., and Post, R. M. (1978) Kindling as a model for alcohol withdrawal syndromes, Br J Psychiatry 133, 1–14.
Brown, M. E., Anton, R. F., Malcolm, R., and Ballenger, J. C. (1988) Alcohol detoxification and withdrawal seizures: clinical support for a kindling hypothesis, Biol Psychiatry 23, 507–514.
Malcolm, R., Roberts, J. S., Wang, W., Myrick, H., and Anton, R. F. (2000) Multiple previous detoxifications are associated with less responsive treatment and heavier drinking during an index outpatient detoxification, Alcohol 22, 159–164.
Chu, K., Koob, G. F., Cole, M., Zorrilla, E. P., and Roberts, A. J. (2007) Dependence-induced increases in ethanol self-administration in mice are blocked by the CRF1 receptor antagonist antalarmin and by CRF1 receptor knockout, Pharmacol Biochem Behav 86, 813–821.
Lee, S., Schmidt, D., Tilders, F., Cole, M., Smith, A., and Rivier, C. (2000) Prolonged exposure to intermittent alcohol vapors blunts hypothalamic responsiveness to immune and non-immune signals, Alcohol Clin Exp Res 24, 110–122.
Becker, H. C., and Hale, R. L. (1993) Repeated episodes of ethanol withdrawal potentiate the severity of subsequent withdrawal seizures: an animal model of alcohol withdrawal “kindling”, Alcohol Clin Exp Res 17, 94–98.
Griffin, W. C., 3rd, Lopez, M. F., and Becker, H. C. (2009) Intensity and duration of chronic ethanol exposure is critical for subsequent escalation of voluntary ethanol drinking in mice, Alcohol Clin Exp Res 33, 1893–1900.
Frye, G. D. a. E., FW (1975) Audiogenic evaluation of withdrawal excitability in rats after chronic ethanol feeding. Pharmacologist 17, 197, 1975., Pharmacologist 17, 1.
McCown, T. J., and Breese, G. R. (1990) Multiple withdrawals from chronic ethanol “kindles” inferior collicular seizure activity: evidence for kindling of seizures associated with alcoholism, Alcohol Clin Exp Res 14, 394–399.
Wills, T. A., Knapp, D. J., Overstreet, D. H., and Breese, G. R. (2008) Differential dietary ethanol intake and blood ethanol levels in adolescent and adult rats: effects on anxiety-like behavior and seizure thresholds, Alcohol Clin Exp Res 32, 1350–1360.
Wills, T. A., Knapp, D. J., Overstreet, D. H., and Breese, G. R. (2010) Interactions of stress and CRF in ethanol-withdrawal induced anxiety in adolescent and adult rats, Alcohol Clin Exp Res 34, 1603–1612.
Janis, G. C., Devaud, L. L., Mitsuyama, H., and Morrow, A. L. (1998) Effects of chronic ethanol consumption and withdrawal on the neuroactive steroid 3alpha-hydroxy-5alpha-pregnan-20-one in male and female rats, Alcohol Clin Exp Res 22, 2055–2061.
Morris, S. A., Kelso, M. L., Liput, D. J., Marshall, S. A., and Nixon, K. (2010) Similar withdrawal severity in adolescents and adults in a rat model of alcohol dependence, Alcohol 44, 89–98.
McCown, T. J., Greenwood, R. S., Frye, G. D., and Breese, G. R. (1984) Electrically elicited seizures from the inferior colliculus: a potential site for the genesis of epilepsy?, Exp Neurol 86, 527–542.
McCown, T. J., Duncan, G. E., Johnson, K. B., and Breese, G. R. (1995) Metabolic and functional mapping of the neural network subserving inferior collicular seizure generalization, Brain Res 701, 117–128.
Paxinos, G., and Watson, C. (1986) The Rat Brain in Stereotaxic Coordinates, Academic Press, New York.
Navarro, M., Cubero, I., Knapp, D. J., and Thiele, T. E. (2003) MTII-induced reduction of voluntary ethanol drinking is blocked by pretreatment with AgRP-(83–132), Neuropeptides 37, 338–344.
Overstreet, D. H., Knapp, D. J., and Breese, G. R. (2002) Accentuated decrease in social interaction in rats subjected to repeated ethanol withdrawals, Alcohol Clin Exp Res 26, 1259–1268.
Majchrowicz, E. (1975) Induction of physical dependence upon ethanol and the associated behavioral changes in rats, Psychopharmacologia 43, 245–254.
Knapp, D. J., and Crews, F. T. (1999) Induction of cyclooxygenase-2 in brain during acute and chronic ethanol treatment and ethanol withdrawal, Alcohol Clin Exp Res 23, 633–643.
Knapp, D. J., Saiers, J. A., and Pohorecky, L. A. (1993) Observations of novel behaviors as indices of ethanol withdrawal-induced anxiety, Alcohol Alcohol Suppl 2, 489–493.
Jarvis, M. F., and Becker, H. C. (1998) Single and repeated episodes of ethanol withdrawal increase adenosine A1, but not A2A, receptor density in mouse brain, Brain Res 786, 80–88.
Kampov-Polevoy, A. B., Matthews, D. B., Gause, L., Morrow, A. L., and Overstreet, D. H. (2000) P rats develop physical dependence on alcohol via voluntary drinking: changes in seizure thresholds, anxiety, and patterns of alcohol drinking, Alcohol Clin Exp Res 24, 278–284.
Overstreet, D. H., Knapp, D. J., and Breese, G. R. (2007) Drug challenges reveal differences in mediation of stress facilitation of voluntary alcohol drinking and withdrawal-induced anxiety in alcohol-preferring P rats, Alcohol Clin Exp Res 31, 1473–1481.
Brick, J. (2006) Standardization of alcohol calculations in research, Alcohol Clin Exp Res 30, 1276–1287.
Acknowledgment
This work was supported in part by AA017462 and AA011605 from NIAAA.
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Knapp, D.J., Breese, G.R. (2012). Models of Chronic Alcohol Exposure and Dependence. In: Kobeissy, F. (eds) Psychiatric Disorders. Methods in Molecular Biology, vol 829. Humana Press. https://doi.org/10.1007/978-1-61779-458-2_13
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DOI: https://doi.org/10.1007/978-1-61779-458-2_13
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