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Bone Research Protocols pp 31–41Cite as

Rat Osteoblast Cultures

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 816))

Abstract

This chapter describes the isolation, culture and staining of primary osteoblasts from the calvaria and long bones of neonatal rats. The key advantages of this assay are that it allows direct measurement of bone matrix deposition and mineralisation, as well as yielding good quantities of osteoblasts at defined stages of differentiation for molecular and histological analysis. A special focus of this chapter is on the role of β-glycerophosphate in cell-mediated mineralisation in these cultures.

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References

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Acknowledgment

Grant Funding: The authors gratefully acknowledge the support of Arthritis Research UK, the Biotechnology and Biological Sciences Research Council (UK) and the European Union (Framework 7 programme).

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Authors and Affiliations

  1. Department of Cell and Developmental Biology, University College London, London, UK

    Isabel R. Orriss & Timothy R. Arnett

  2. Department of Cell and Developmental Biology, University of Lancaster, Lancaster, UK

    Sarah E. B. Taylor

Authors
  1. Isabel R. Orriss
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  2. Sarah E. B. Taylor
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  3. Timothy R. Arnett
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Corresponding author

Correspondence to Isabel R. Orriss .

Editor information

Editors and Affiliations

  1. Institute of Medical Sciences, University of Aberdeen, Forester Hill, Aberdeen, AB25 2ZD, United Kingdom

    Miep H. Helfrich

  2. Institute of Genetics and Molecular Medi, Rheumatic Diseases Unit, University of Edinburgh, Crewe Road South, Edinburgh, EH4 2XU, United Kingdom

    Stuart H. Ralston

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© 2012 Springer Science+Business Media, LLC

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Orriss, I.R., Taylor, S.E.B., Arnett, T.R. (2012). Rat Osteoblast Cultures. In: Helfrich, M., Ralston, S. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 816. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-415-5_3

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  • DOI: https://doi.org/10.1007/978-1-61779-415-5_3

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-414-8

  • Online ISBN: 978-1-61779-415-5

  • eBook Packages: Springer Protocols

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