Abstract
Sensitive and quantitative analysis of proteins is central to disease diagnosis, drug screening, and proteomic studies. Among recent research advances exploiting new nanomaterials for biomolecule analysis, silicon nanowires (SiNWs), which are configured as field-effect transistors (FETs), have emerged as one of the most promising and powerful platforms for label-free, real-time, and highly sensitive electrical detection of proteins as well as many other biological species. Here, we describe a detailed protocol for realizing SiNW biosensors for protein detection that includes SiNW synthesis, FET device fabrication, surface receptor functionalization, and electrical sensing measurements. Moreover, incorporating both p-type and n-type SiNWs in the same sensor array provides a unique means of internal control for sensing signal verification.
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References
Morales, A.M., Lieber, C.M. (1998) A laser ablation method for the synthesis of crystalline semiconductor nanowires. Science 279, 208–211.
Lieber, C.M., Wang, Z.L. (2007) Functional nanowires. MRS Bull 32, 99–108.
Patolsky, F., Timko, B.P., Zheng, G., et al. (2007) Nanowire-based nanoelectronic devices in the life sciences. MRS Bull 32, 142–149.
Cui, Y., Wei, Q., Park, H., et al. (2001) Nanowire nanosensors for highly sensitive and selective detection of biological and chemical species. Science 293, 1289–1292.
Zheng, G., Patolsky, F., Cui, Y., et al. (2005) Multiplexed electrical detection of cancer markers with nanowire sensor arrays. Nat Biotechnol 23, 1294–1301.
Stern, E., Klemic, J.F., Routenberg, D.A., et al. (2007) Label-free immunodetection with CMOS-compatible semiconducting nanowires. Nature 445, 519–522.
McAlpine, M.C., Ahmad, H., Wang, D.W., et al. (2007) Highly ordered nanowire arrays on plastic substrates for ultrasensitive flexible chemical sensors. Nat Mater 6, 379–384.
Park, I., Li Z., Pisano, A.P., et al. (2010) Top-down fabricated silicon nanowire sensors for real-time chemical detection. Nanotechnol 21, 015501.
Domansky, K., Janata, J. (1993) Present state of fabrication of chemically sensitive field-effect transistors. Analyst 118, 335–340.
Janata, J. (1994) 20 Years of ion-selective field-effect transistors. Analyst 119, 2275–2278.
Sze, S.M., Ng., K.K. (1981) Physics of Semiconductor Devices, 2nd ed. Wiley, New York.
Srinivas, P.R., Kramer, B.S., Srivastava, S. (2001) Trends in biomarker research for cancer detection. Lancet Oncol 2, 698–704.
Etzioni, R. Urban, N., Ramsey, S., et al. (2003) The case for early detection. Nat Rev Cancer 3, 243–252.
Ward, A.M., Catto, J.W.F., Hamdy, F.C. (2001) Prostate specific antigen: biology, biochemistry and available commercial assays. Ann Clin Biochem 38, 633–651.
Patolsky, F., Zheng, G., Lieber, C.M. (2006) Fabrication of silicon nanowire devices for ultrasensitive, label-free, real-time detection of biological and chemical species. Nat Protocol 1, 1711–1724.
Whitesides, G.M., Ostuni, E., Takayama, S. et al. (2001) Soft lithography in biology and biochemistry. Annu Rev Biomed Eng 3, 335.
Acknowledgments
Gengfeng Zheng, acknowledges the support of this work by the National Natural Science Foundation of China (21071033), the Program for New Century Excellent Talents in University (NCET-10-0357), the Shanghai Pujiang Program (10PJ1401000), and Fudan University startup fund. Charles M. Lieber, acknowledges the support of this work by the NIH R21 award (5R21CA133519) and the Director’s Pioneer Award (5DP1OD003900).
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Zheng, G., Lieber, C.M. (2011). Nanowire Biosensors for Label-Free, Real-Time, Ultrasensitive Protein Detection. In: Toms, S., Weil, R. (eds) Nanoproteomics. Methods in Molecular Biology, vol 790. Humana Press. https://doi.org/10.1007/978-1-61779-319-6_18
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DOI: https://doi.org/10.1007/978-1-61779-319-6_18
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