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Gene Expression Profiling pp 123–137Cite as

2D Gel Electrophoresis and Mass Spectrometry Identification and Analysis of Proteins

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 784))

Abstract

Analysis of the protein expression patterns in clinical samples and cells by proteomic technologies offers opportunities to discover potentially new biomarkers for early detection and diagnosis of disease. One of the most widely used techniques to study the proteome of a biological system is two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). In particular, a modified version of 2D-PAGE, two-dimensional difference gel electrophoresis (2D-DIGE), which uses differential labelling of protein samples with up to three fluorescent tags, offers greater sensitivity and reproducibility over conventional 2D-PAGE. In this chapter, we will introduce methods for the analysis of whole cell lysates from human cancer cell lines using 2D-DIGE and identification of differentially expressed proteins using liquid chromatography mass spectrometry, i.e. LC–MS/MS.

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Authors and Affiliations

  1. National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland

    Paula Meleady

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  1. Paula Meleady
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Correspondence to Paula Meleady .

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Editors and Affiliations

  1. School of Pharmacy, and Pharmaceutical Sciences, Trinity College Dublin, East End Development 4/5, Dublin, 2, Ireland

    Lorraine O'Driscoll

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Meleady, P. (2011). 2D Gel Electrophoresis and Mass Spectrometry Identification and Analysis of Proteins. In: O'Driscoll, L. (eds) Gene Expression Profiling. Methods in Molecular Biology, vol 784. Humana Press. https://doi.org/10.1007/978-1-61779-289-2_9

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  • DOI: https://doi.org/10.1007/978-1-61779-289-2_9

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-288-5

  • Online ISBN: 978-1-61779-289-2

  • eBook Packages: Springer Protocols

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