Abstract
The directed differentiation of human pluripotent stem cells into specific, determined, and high-purity cell types can provide a means to study the cellular and molecular mechanisms of development and to generate cells for potential therapeutic applications. The ability to derive homogeneous cell populations obviates the need for transgene expression or cell sorting methods and can improve selection efficiency, lineage differentiation, cell viability, and clinical utility. Compared to undifferentiated pluripotent stem cells, high-purity cell phenotypes for clinical therapeutic strategies are expected to enhance engraftment, potentiate clinical efficacy, and decrease the risk of adverse effects such as dedifferentiation or teratoma formation. Clinical interest in the derivation of oligodendrocyte progenitor cells from pluripotent stem cells is based on research that demonstrates the effectiveness of progenitor cell transplants to improve outcomes after spinal cord injury. Here, we describe a protocol to generate oligodendroglial lineage-specific cells in high purity from human embryonic stem cells.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Sharp J, Keirstead HS (2007). Therapeutic applications of oligodendrocyte precursors derived from human embryonic stem cells. Curr Opin Biotechnol. 18: 434–440.
Nistor GI, Totoiu MO, Haque N, Carpenter MK, Keirstead HS (2005). Human embryonic stem cells differentiate into oligodendrocytes in high purity and remyelinate after spinal cord transplantation. Glia 49: 385–396.
Izrael M, Zhang P, Kaufman R, Shinder V, Ella R, Amit M, Itskovitz-Eldor J, Chebath J, Revel M (2007). Human oligodendrocytes derived from embryonic stem cells: Effect of noggin on phenotypic differentiation in vitro and on myelination in vivo. Mol Cell Neurosci. 34: 310–323.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Sharp, J., Hatch, M., Nistor, G., Keirstead, H. (2011). Derivation of Oligodendrocyte Progenitor Cells from Human Embryonic Stem Cells. In: Schwartz, P., Wesselschmidt, R. (eds) Human Pluripotent Stem Cells. Methods in Molecular Biology, vol 767. Humana Press. https://doi.org/10.1007/978-1-61779-201-4_29
Download citation
DOI: https://doi.org/10.1007/978-1-61779-201-4_29
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61779-200-7
Online ISBN: 978-1-61779-201-4
eBook Packages: Springer Protocols