Osteogenic Differentiation of Human Multipotent Mesenchymal Stromal Cells

Gupta, Deepak M.; Panetta, Nicholas J.; Longaker, Michael T.

doi:10.1007/978-1-60761-999-4_16

Deepak M. Gupta,
Nicholas J. Panetta &
Michael T. Longaker⁴

Part of the book series: Methods in Molecular Biology ((MIMB,volume 698))

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Abstract

A comprehensive knowledge of the molecular biology underlying osteogenic differentiation in a controlled, laboratory setting may promise optimization of future cell-based tissue engineering strategies for clinical problems. The scope of this review encompasses a discussion of the methodology utilized to perform such studies. Our laboratory routinely performs both in vitro and in vivo assays underlying osteogenic differentiation, and the widespread use of singular methodology across multiple investigators and institutions promises great advancements for the skeletal tissue engineering community.

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Reference

Cowan, C.M., Shi, Y.Y., Aalami, O.O. et al. Adipose-derived adult stromal cells heal critical-size mouse calvarial defects. Nat Biotechnol 22, 560–7 (2004).
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Acknowledgments

This work was supported by NIH R01 DE014526, NIH R21 DE019274, and grants from the Oak and Hagey Foundations to M.T.L., NIH F32 AR055871, Stanford University Dean’s Award and Lucile Packard Children’s Health Research Program Award to D.M.G., and an American College of Surgeons Resident Research Award and Lucile Packard Children’s Health Research Program Award to N.J.P.

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Authors and Affiliations

Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA, USA
Michael T. Longaker

Authors

Deepak M. Gupta
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Nicholas J. Panetta
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Michael T. Longaker
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Corresponding author

Correspondence to Michael T. Longaker .

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Editors and Affiliations

, Primary and Stem Cell Systems, Life Technologies, Frederick, 21704, USA
Mohan Vemuri
Cellular Dynamics International, Inc., Science Drive 525, Madison, 53711, Wisconsin, USA
Lucas G. Chase
Life Technologies, Executive Way 7335, Frederick, 21704, Maryland, USA
Mahendra S. Rao

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Gupta, D.M., Panetta, N.J., Longaker, M.T. (2011). Osteogenic Differentiation of Human Multipotent Mesenchymal Stromal Cells. In: Vemuri, M., Chase, L., Rao, M. (eds) Mesenchymal Stem Cell Assays and Applications. Methods in Molecular Biology, vol 698. Humana Press. https://doi.org/10.1007/978-1-60761-999-4_16

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DOI: https://doi.org/10.1007/978-1-60761-999-4_16
Published: 11 February 2011
Publisher Name: Humana Press
Print ISBN: 978-1-60761-998-7
Online ISBN: 978-1-60761-999-4
eBook Packages: Springer Protocols

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