Abstract
The tachykinins represent the largest known peptide family and are responsible for a range of pleiotropic functions in both vertebrates and invertebrates. Recent research has shown a diversity of mechanisms such as mRNA splicing, precursor processing and post-translation modification that can lead to a complex and continually expanding repertoire of tachykinin peptides. The peptidomic analysis of the tachykinins has been hindered by the lack of specific methodologies to capture, purify and characterise each tachykinin. This chapter summarises some of the methods that have been developed in order to further purify and characterise individual groups of tachykinin peptides from the peptidome.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Severini, C., Improta, G., Falconieri-Erspamer, G., Salvadori, S., and Erspamer, V. (2002) The tachykinin peptide family. Pharmacol. Rev. 54, 285–322.
Page, N.M. (2005) New challenges in the study of the mammalian tachykinins. Peptides. 26, 1356–1368.
Page, N.M., Bell, N.J., Gardiner, S.M., Manyonda, I.T., Brayley, K.J., Strange, P.G., and Lowry, P.J. (2003) Characterization of the endokinins: human tachykinins with cardiovascular activity.. Proc. Natl. Acad. Sci. USA. 100, 6245–6250.
Kurtz, M.M., Wang, R., Clements, M.K., Cascieri, M.A., Austin, C.P., Cunningham, B.R., Chicchi, G.G., and Liu, Q. (2002) Identification, localization and receptor characterization of novel mammalian substance P-like peptides. Gene. 296, 205–212.
Page, N.M. (2004) Hemokinins and endokinins. Cell Mol. Life Sci. 61, 1652–1663.
Acknowledgments
The Medical Research Council (UK) and the Biomedical and Pharmaceutical Sciences Research Group (Kingston University London) supported this work.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Page, N.M., Weston-Bell, N.J. (2010). Immunochemical Methods for the Peptidomic Analysis of Tachykinin Peptides and Their Precursors. In: Soloviev, M. (eds) Peptidomics. Methods in Molecular Biology, vol 615. Humana Press. https://doi.org/10.1007/978-1-60761-535-4_22
Download citation
DOI: https://doi.org/10.1007/978-1-60761-535-4_22
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-60761-534-7
Online ISBN: 978-1-60761-535-4
eBook Packages: Springer Protocols