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Isolation and Culture of Hair Follicle Pluripotent Stem (hfPS) Cells and Their Use for Nerve and Spinal Cord Regeneration

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Epidermal Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 585))

Abstract

The hair follicle is dynamic, cycling between growth (anagen), regression (catagen), and resting (telogen) phases throughout life. We have demonstrated that nestin-expressing hair follicle stem cells give rise to follicle structures during early anagen or growth phase of the hair follicle. Nestin-expressing hair follicle stem cells appear in the hair follicular stem cell area, the permanent upper hair follicle immediately below the sebaceous glands and above the bulge area. The nestin-expressing hair follicle stem cells can differentiate into neurons, glia, keratinocytes, smooth muscle cells, and melanocytes in vitro. Furthermore, the hair follicle stem cells promote the recovery of peripheral nerve and spinal cord injury. We have termed these cells hair follicle pluripotent stem (hfPS) cells. These results suggest that hfPS cells provide an important accessible, autologous source of adult stem cells with potential for use in regenerative medicine.

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Author information

Authors and Affiliations

  1. AntiCancer, Inc., San Diego, CA, USA

    Yasuyuki Amoh & Robert M. Hoffman

  2. Department of Dermatology, Kitasato University School of Medicine, Sagamihara, Japan

    Yasuyuki Amoh

  3. Department of Surgery, University of California, San Diego, CA, USA

    Robert M. Hoffman

Authors
  1. Yasuyuki Amoh
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  2. Robert M. Hoffman
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Editor information

Editors and Affiliations

  1. Sprott Centre for Stem Cell Research, Ottawa Hosp. Research Institute (OHRI), Smyth Road 501, Ottawa, K1H 8L6, Canada

    Kursad Turksen

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© 2010 Humana Press, a part of Springer Science+Business Media, LLC

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Amoh, Y., Hoffman, R.M. (2010). Isolation and Culture of Hair Follicle Pluripotent Stem (hfPS) Cells and Their Use for Nerve and Spinal Cord Regeneration. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology, vol 585. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-380-0_28

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  • DOI: https://doi.org/10.1007/978-1-60761-380-0_28

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-379-4

  • Online ISBN: 978-1-60761-380-0

  • eBook Packages: Springer Protocols

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