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Protocols for Adult Stem Cells pp 103–116Cite as

Viral Packaging and Transduction of Adult Hippocampal Neural Progenitors

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 621))

Abstract

Genetic manipulation of adult hippocampal neural progenitor cells is a useful technique for exploring gene function through gain of function and loss of function mutations or RNAi. Furthermore, the introduction of new genes can “re-program” progenitor cell behavior to force a desired lineage in signaling environments that are not normally permissive for that cell fate. Additionally, by using a systems biology approach, neural progenitors can even be taught new behaviors and responses to signaling. In this chapter, we describe protocols for retroviral and lentiviral packaging and transduction of progenitors. These techniques are important for studying the role of various genes in progenitor fate choice.

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References

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Acknowledgment

We acknowledge Matthew Robertson for critical reading of the manuscript. This work was funded by an NSF Graduate Research Fellowship (to JP), a California Institute for Regenerative Medicine Training Grant (T1-00007), and NSF BES-0629202.

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Authors and Affiliations

  1. Department of Chemical Engineering, University of California, Berkeley, CA, USA

    Joseph Peltier & David V. Schaffer

Authors
  1. Joseph Peltier
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  2. David V. Schaffer
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Corresponding author

Correspondence to David V. Schaffer .

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Editors and Affiliations

  1. Dept. Bioengineering, University of California, Berkeley, Evans Hall 479, Berkeley, 94720-1762, USA

    Irina M. Conboy

  2. Helen Wills Neuroscience Inst., University of California, Berkeley, Stanley Hall 176, Berkeley, 94720-3220, USA

    David V. Schaffer

  3. Lawrence Berkeley National Lab., Dept. Cancer Biology, University of California, Berkeley, Cyclotron Rd. 1, Berkeley, 94720, USA

    Mary Helen Barcellos-Hoff

  4. Dept. Bioengineering, University of California, Berkeley, Stanley Hall, Berkeley, 94720-1762, USA

    Song Li

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Peltier, J., Schaffer, D.V. (2010). Viral Packaging and Transduction of Adult Hippocampal Neural Progenitors. In: Conboy, I., Schaffer, D., Barcellos-Hoff, M., Li, S. (eds) Protocols for Adult Stem Cells. Methods in Molecular Biology™, vol 621. Humana Press. https://doi.org/10.1007/978-1-60761-063-2_7

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  • DOI: https://doi.org/10.1007/978-1-60761-063-2_7

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-062-5

  • Online ISBN: 978-1-60761-063-2

  • eBook Packages: Springer Protocols

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