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Biosensors and Biodetection pp 179–187Cite as

Phase Sensitive Interferometry for Biosensing Applications

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 503))

Summary

A simple yet highly sensitive implementation of an interferometric technique for a label-free molecular biosensing application is described. The intereferometric detection method is based on the phase-sensitive detection of spectral interference fringes. The change in optical path length due to binding of biomolecules on functionalized optically clear substrates can be quantified by detecting the change in the phase of the spectral fringes. The common path interferometeric design permits measurement of sub- monolayer binding of biomolecules to the sensor surfaces.

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Author information

Authors and Affiliations

  1. University of Texas at Arlington, Arlington, TX, USA

    Digant P. Davé

Authors
  1. Digant P. Davé
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Editor information

Editors and Affiliations

  1. FDA Center for Devices and Radiological Health, Silver Spring, MD, USA

    Avraham Rasooly

  2. National Cancer Institute, Bethesda, MD, USA

    Avraham Rasooly

  3. Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA

    Keith E. Herold

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC, a part of Springer Science+Business Media, LLC

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Cite this protocol

Davé, D.P. (2009). Phase Sensitive Interferometry for Biosensing Applications. In: Rasooly, A., Herold, K.E. (eds) Biosensors and Biodetection. Methods in Molecular Biology™, vol 503. Humana Press. https://doi.org/10.1007/978-1-60327-567-5_9

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  • DOI: https://doi.org/10.1007/978-1-60327-567-5_9

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-566-8

  • Online ISBN: 978-1-60327-567-5

  • eBook Packages: Springer Protocols

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