Abstract
RNA interference is a phenomenon in which specific, endogenous genes are silenced by mRNA degradation. This technology is highly regarded as a potential therapeutic due to its high efficacy and low toxicity. However, the difficulty of delivering RNAi to target cells has impeded the development of RNAi-based therapies. One method to overcome this barrier is the use of a nonpathogenic bacteria vector, Escherichia coli, to deliver RNAi to target cells with high efficacy. In transkingdom interference RNAi (tkRNAi) delivery, E. coli were engineered to transcribe short RNA (shRNA) from a plasmid (TRIP) containing the invasin gene Inv and the listeriolysin O gene Hly. tkRNAi is successful in eliciting efficient gene silencing in vitro and in vivo.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Nguyen, T.A., Fruehauf, J.H. (2009). transkingdom RNA Interference (tkRNAi): A Novel Method to Induce Therapeutic Gene Silencing. In: De Libero, G. (eds) T Cell Protocols. Methods in Molecular Biology™, vol 514. Humana Press. https://doi.org/10.1007/978-1-60327-527-9_3
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DOI: https://doi.org/10.1007/978-1-60327-527-9_3
Publisher Name: Humana Press
Print ISBN: 978-1-58829-587-3
Online ISBN: 978-1-60327-527-9
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