Abstract
Mitochondrial dysfunction has been increasingly shown as a critical process that makes certain areas of the brain more susceptible not only to neurological disease but also to aging. Quantitative histochemistry is a series of procedures for measuring select metabolites in discrete regions of the brain, as they exist in vivo. The development of this method has been useful in establishing energy imbalance following ischemia but more recently has become useful in studying those processes related to the mitochondria which make the brain more susceptible to a variety of neurological insults. The relatively inexpensive cost to assay a given brain metabolite makes this methodology useful in the interpretation of molecular and biochemical responses in terms of the condition of the tissue following a neurological insult.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Greengard P (1956) Determination of intermediary metabolites by enzymic fluorimetry. Nature 178: 632–4.
Lowry OH, Passonneau JV (1972) A Flexible System of Enzymatic Analysis. New York: Academic Press.
Lust WD, Feussner GK, Barbehenn EK, Passonneau JV (1981) The enzymatic measurement of adenine nucleotides and P-creatine in picomole amounts. Anal. Biochem. 110: 258–66.
Lundin A, Richardsson A, Thore A (1976) Continuous monitoring of ATP-converting reactions by purified firefly luciferase. Anal. Biochem. 75: 611–20.
Siesjo BK (1978) Brain energy metabolism and catecholaminergic activity in hypoxia, hypercapnia and ischemia. J. Neural Transm. Suppl. 17–22.
Lenox RH, Kant GH, Meyerhoff JL (1982) Rapid enzyme inactivation. In: Lajtha A, ed. Handbook of Neurochemistry. New York: Plenum, pp. 77–102.
Lust WD, Passonneau JV, Veech RL (1973) Cyclic adenosine monophosphate, metabolites, and phosphorylase in neural tissue: a comparison a methods of fixation. Science 181: 280–2.
Lust WD, Murakami N, de Azeredo F, Passonneau JV (1980) A comparison of methods for brain fixation. In: Passonneau JV, Hawkins RA, Lust WD, Welsh RA, eds. Cerebral Metabolism and Neural Function. Baltimore: Williams and Wilkins, pp. 10-9.
Lust WD, Ricci AJ, Selman WR, Ratcheson RA (1989) Methods of fixation of nervous tissue for use in the study of cerebral energy metabolism. In: Boulton, AA; Baker, GB; Butterworth, RF, eds. Carbohydrates and Energy Metabolism. Totowa, NJ: Humana Press, pp. 1–42.
Goldberg ND, Passonneau JV, Lowry OH (1966) Effects of changes in brain metabolism on the levels of citric acid cycle intermediates. J. Biol. Chem. 241: 3997–4003.
Ponten U, Ratcheson RA, Salford LG, Siesjo BK (1973) Optimal freezing conditions for cerebral metabolites in rats. J. Neurochem. 21: 1127–38.
Mrsulja BB, Ueki Y, Lust WD (1986) Regional metabolite profiles in early stages of global ischemia in the gerbil. Metab. Brain Dis. 1: 205–20.
Harik SI, al Mudallal AS, LaManna JC, Lust WD, Levin BE (1997) Ketogenic diet and the brain. Ann. N. Y. Acad. Sci. 835: 218–24.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 2007 Humana Press Inc.
About this protocol
Cite this protocol
Zechel, J., Lust, W.D., Puchowicz, M. (2007). Biochemical Methods to Assess the Coupling of Brain Energy Metabolism in Control and Disease States. In: Borsello, T. (eds) Neuroprotection Methods and Protocols. Methods in Molecular Biology, vol 399. Humana Press. https://doi.org/10.1007/978-1-59745-504-6_7
Download citation
DOI: https://doi.org/10.1007/978-1-59745-504-6_7
Publisher Name: Humana Press
Print ISBN: 978-1-58829-666-5
Online ISBN: 978-1-59745-504-6
eBook Packages: Springer Protocols