Abstract
A considerable amount of work has been done during the last 20 yr to genetically enhance the efficacy of baculovirus insecticides. Following construction of a genetically altered baculovirus, laboratory bioassays are used to quantify various parameters of insecticidal activity such as the median lethal concentration (or dose) required to kill 50% of infected larvae (LC50 or LD50), median survival time of larvae infected at a fixed dose (ST50), and feeding damage incurred by infected larvae. In this chapter, protocols are described for a variety of bioassays and corresponding data analyses for assessment of the insecticidal activity or host range of baculovirus insecticides. Methods are also provided for baculovirus inoculation of larvae using a microapplicator for determining ST50 or for examining physiological effects.
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Li, H., Bonning, B.C. (2007). Evaluation of the Insecticidal Efficacy of Wild-Type and Recombinant Baculoviruses. In: Murhammer, D.W. (eds) Baculovirus and Insect Cell Expression Protocols. Methods in Molecular Biology™, vol 388. Humana Press. https://doi.org/10.1007/978-1-59745-457-5_20
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DOI: https://doi.org/10.1007/978-1-59745-457-5_20
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