Summary
Gas-phase fractionation (GPF), defined as iterative mass spectrometric interrogations of a sample over multiple smaller mass-to-charge (m/z) ranges, enables the ions selected for collision-induced dissociation to come from a greater number of unique peptides compared to the ions selected from the wide mass range scan in automated liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. GPF is described as a means to achieve higher proteome coverage than multiple LC-MS/MS analyses of unfractionated complex peptide mixtures. It is applied to organellar proteomics through analysis of yeast peroxisomal proteins obtained from a discontinuous Nycodenz gradient fraction known to be enriched with yeast peroxisomal membrane proteins.
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References
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Kennedy, J., Yi, E.C. (2008). Use of Gas-Phase Fractionation to Increase Protein Identifications. In: Pflieger, D., Rossier, J. (eds) Organelle Proteomics. Methods in Molecular Biology™, vol 432. Humana Press. https://doi.org/10.1007/978-1-59745-028-7_15
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DOI: https://doi.org/10.1007/978-1-59745-028-7_15
Publisher Name: Humana Press
Print ISBN: 978-1-58829-779-2
Online ISBN: 978-1-59745-028-7
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