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  • © 2010

Antibody Engineering Volume 1

  • The updated second edition of the successful Springer Lab Manual "Antibody Engineering" is now offered in two volumes with nearly the double number of protocols
  • With both new and well established techniques
  • Detailed step-by-step protocols including all materials and practical advices
  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Protocols Handbooks (SPH)

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Softcover Book USD 279.99
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Table of contents (50 protocols)

  1. Front Matter

    Pages i-xiii
  2. Cloning of the Antigen-binding Site from Hybridoma

    1. Front Matter

      Pages 1-1
    2. Cloning of Variable Domains from Mouse Hybridoma by PCR

      • Nina Strebe, Frank Breitling, Dieter Moosmayer, Bodo Brocks, Stefan Dübel
      Pages 3-14
    3. Coning Hybridoma cDNA by RACE

      • Andrew Bradbury
      Pages 15-20
    4. Construction of scFv Fragments from Hybridoma or Spleen Cells by PCR Assembly

      • Jonas V. Schaefer, Annemarie Honegger, Andreas Plückthun
      Pages 21-44
  3. Generation of Antibody Repertoires

    1. Front Matter

      Pages 43-43
    2. Mouse Immune Libraries for the Generation of ScFv Fragments Directed Against Human Cell Surface Antigens

      • Christian Kellner, Sahar Mohseni Nodehi, Matthias Peipp
      Pages 47-63
    3. Human Antibody Gene Libraries

      • Michael Hust, Stefan Dübel
      Pages 65-84
    4. Synthetic Antibody Libraries

      • Pierre Martineau
      Pages 85-97
    5. Immune Libraries from Nonhuman Primates (NHP)

      • Thibaut Pelat, Michael Hust, Philippe Thullier
      Pages 99-114
    6. Generation of Rabbit Immune Libraries

      • Rüdiger Ridder, Hermann Gram
      Pages 115-123
  4. Selection of Antibody Fragments from Combinatorial Libraries

    1. Front Matter

      Pages 128-128
    2. Immunotube Selections

      • Roland E. Kontermann
      Pages 127-137
    3. Phage Display and Selection in Microtitre Plates

      • Michael Hust, Michael Mersmann
      Pages 139-149
    4. Phage Display and Selections on Biotinylated Antigens

      • Patrick Chames, Daniel Baty
      Pages 151-164
    5. Phage Display and Subtractive Selection on Cells

      • Steffen U. Eisenhardt, Karlheinz Peter
      Pages 165-182
    6. Improving Phage Display Throughput by Using Hyperphage, Miniaturized Titration and pVIII (g8p) ELISA

      • Frank Breitling, Olaf Broders, Saskia Helmsing, Michael Hust, Stefan Dübel
      Pages 197-206
    7. Yeast Display and Selections

      • Kelly Davis Orcutt, K. Dane Wittrup
      Pages 207-233
    8. The Generation of Transgenic Mice Expressing Human Antibody Repertoires

      • Xiangang Zou, Guocheng Lan, Michael J. Osborn, Marianne Brüggemann
      Pages 235-254

About this book

Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development.

Editors and Affiliations

  • (Biomedical Engineering) Institut für Zellbiologie und Immunologie, Universität Stuttgart, Stuttgart, Germany

    Roland Kontermann

  • Technische Universität Braunschweig, Institut für Biochemie und Biotechnologie, Germany

    Stefan Dübel

Bibliographic Information

  • Book Title: Antibody Engineering Volume 1

  • Editors: Roland Kontermann, Stefan Dübel

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-3-642-01144-3

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer-Verlag GmbH Germany, part of Springer Nature 2010

  • Hardcover ISBN: 978-3-642-01143-6Published: 26 March 2010

  • Softcover ISBN: 978-3-662-49594-0Published: 26 March 2010

  • eBook ISBN: 978-3-642-01144-3Published: 10 March 2010

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 2

  • Number of Pages: XIII, 788

  • Additional Information: Originally published in one volume, within the series "Springer Lab Manuals"

  • Topics: Immunology, Antibodies, Biotechnology

Buy it now

Buying options

eBook USD 219.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 279.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 379.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access