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  • Book
  • © 2015

Methods in Mouse Atherosclerosis

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1339)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiv
  2. Use of Mouse Models in Atherosclerosis Research

    • Godfrey S. Getz, Catherine A. Reardon
    Pages 1-16
  3. Cytokines and Immune Responses in Murine Atherosclerosis

    • Pascal J. H. Kusters, Esther Lutgens
    Pages 17-40
  4. Intravital Microscopy for Atherosclerosis Research

    • Remco T. A. Megens, Oliver Soehnlein
    Pages 41-60
  5. Noninvasive Molecular Imaging of Mouse Atherosclerosis

    • Martina A. McAteer, Robin P. Choudhury
    Pages 61-83
  6. Isolation, Culture, and Polarization of Murine Bone Marrow-Derived and Peritoneal Macrophages

    • Inés Pineda-Torra, Matthew Gage, Alba de Juan, Oscar M. Pello
    Pages 101-109
  7. Isolation and Culture of Aortic Smooth Muscle Cells and In Vitro Calcification Assay

    • Ricardo Villa-Bellosta, Magda R. Hamczyk
    Pages 119-129
  8. Immunostaining of Lymphocytes in Mouse Atherosclerotic Plaque

    • Anton Gisterå, Daniel F. J. Ketelhuth
    Pages 149-159
  9. Flow Cytometric Analysis of Immune Cells Within Murine Aorta

    • Breanne N. Gjurich, Parésa L. Taghavie-Moghadam, Elena V. Galkina
    Pages 161-175
  10. Quantification of Apoptosis in Mouse Atherosclerotic Lesions

    • Nichola L. Figg, Martin R. Bennett
    Pages 191-199
  11. Quantification of In Vitro Macrophage Cholesterol Efflux and In Vivo Macrophage-Specific Reverse Cholesterol Transport

    • Joan Carles Escolà-Gil, Miriam Lee-Rueckert, David Santos, Lídia Cedó, Francisco Blanco-Vaca, Josep Julve
    Pages 211-233
  12. In Vitro Macrophage Phagocytosis Assay

    • Magda R. Hamczyk, Ricardo Villa-Bellosta, Vicente Andrés
    Pages 235-246
  13. Glucose and Insulin Tolerance Tests in the Mouse

    • Ángela Vinué, Herminia González-Navarro
    Pages 247-254
  14. Pressure Myography to Study the Function and Structure of Isolated Small Arteries

    • Olav L. Schjørring, Rune Carlsson, Ulf Simonsen
    Pages 277-295

About this book

This volume reviews available mouse models of atherosclerosis, basic aspects of disease initiation and progression, and the visualization of atherosclerotic lesions with invasive and non-invasive techniques that are widely used both in basic research and in the clinic. The chapters catalogue a wide range of in vitro and in vivo experimental methods used for atherosclerosis research, including thorough protocols and valuable notes based on the authors’ personal wet lab experience. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Methods in Mouse Atherosclerosis will be a valuable working guide for researchers performing mouse-based atherosclerosis studies.

Editors and Affiliations

  • Laboratory of Molecular and Genetic Cardiovascular Pathophysiology, Centro Nacional de Investigaciones Cardiovascular Carlos III (CNIC), Madrid, Spain

    Vicente Andrés, Beatriz Dorado

Bibliographic Information

  • Book Title: Methods in Mouse Atherosclerosis

  • Editors: Vicente Andrés, Beatriz Dorado

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2929-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2928-3Published: 09 October 2015

  • Softcover ISBN: 978-1-4939-4935-9Published: 23 August 2016

  • eBook ISBN: 978-1-4939-2929-0Published: 07 October 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 400

  • Number of Illustrations: 16 b/w illustrations, 58 illustrations in colour

  • Topics: Molecular Medicine, Cardiology, Animal Models

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access