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Methods in Gut Microbial Ecology for Ruminants

  • Book
  • © 2005

Overview

Editors:
  1. Harinder P.S. Makkar

    1. Animal Production and Health Section Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Vienna, Austria

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  2. Christopher S. McSweeney

    1. CSIRO Livestock Industries, Queensland Bioscience Precinct, St Lucia, Australia

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    You can also search for this editor in PubMed   Google Scholar

  • A comprehensive up-to-date account of the methodologies and protocols for conventional and modern molecular techniques, currently in use for studying the gut microbial ecology of ruminants
  • Written by an expert group of scientists working in the area of ruminant digestion and gut microbiology
  • Written in a recipe-like format designed for direct practical use in the laboratory
  • Only book in the market presenting recent molecular methodologies for studying gut microbial ecology in ruminants

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Table of contents (17 chapters)

  1. Front Matter

    Pages i-xi
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  2. Designing **in vivo microbial ecology studies

    1. Experimental designs for rumen microbiology

      • Adrian R. Egan
      Pages 3-19

  3. Classical methods for isolation, enumeration, cultivation and functional assays of rumen microbes

    1. Rumen bacteria

      • Christopher S. McSweeney, Stuart E. Denman, Roderick I. Mackie
      Pages 23-37

    2. Bacteriophages

      • Athol V. Klieve
      Pages 39-46

    3. Methanogenic archaea

      • Keith N. Joblin
      Pages 47-53

    4. Anaerobic fungi

      • Michael K. Theodorou, Jayne Brookman, Anthony P.J. Trinci
      Pages 55-66

    5. Ciliate protozoa

      • Burk A. Dehority
      Pages 67-78

  4. PCR-based methods for analysis of populations and gene expression

    1. Nucleic acid extraction, oligonucleotide probes and PCR methods

      • Zhongtang Yu, Robert J. Forster
      Pages 81-104

    2. Quantitative (real-time) PCR

      • Stuart E. Denman, Christopher S. McSweeney
      Pages 105-115

  5. Molecular fingerprinting techniques for genotypic analysis of pure cultures and microbial communities

    1. Denaturing gradient gel electrophoresis

      • Svetlana A. Kocherginskaya, Isaac K.O. Cann, Roderick I. Mackie
      Pages 119-128

    2. Bacteriophage populations

      • Athol V. Klieve, Rosalind A. Gilbert
      Pages 129-137

    3. Anaerobic fungal populations

      • Jayne L. Brookman, Matthew J. Nicholson
      Pages 139-150

    4. RAPD, RFLP, T-RFLP, AFLP, RISA

      • Stuart E. Denman, Makoto Mitsumori, Christopher S. Mcsweeney
      Pages 151-159

  6. DNA clone libraries of microbial communities

    1. 16S/18S ribosomal DNA clone library analysis of rumen microbial diversity

      • André-Denis G. Wright, Kiyoshi Tajima, Rustam I. Aminov
      Pages 163-174

  7. Use of small subunit ribosomal RNA directed oligonucleotide probes for microbial population studies

    1. Northern blot analysis to investigate the abundance of microorganisms

      • Denis O. Krause
      Pages 177-190

    2. Whole cell probing with fluorescently labelled probes for in situ analysis of microbial populations

      • Linda L. Blackall
      Pages 191-199

    3. Combined fluorescence in situ hybridization and microautoradiography (FISH-MAR)

      • Maneesha P. Ginige
      Pages 201-206

  8. Genomic analysis of microbial ecosystems

    1. Metagenomic analysis of the microbiomes in ruminants and other herbivores

      • Mark Morrison, Sarah E. Adams, Karen E. Nelson, Graeme T. Attwood
      Pages 209-220

  9. Back Matter

    Pages 221-225
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Keywords

About this book

Asaresultofvarioushumanactivities,suchasincreaseinhumanpopulation,decrease in arable land due to soil degradation, urbanization, industrialization and associated increase in the demand for livestock products, dramatic changes are occurring in the global ruminant livestock sector. These changes includeshift inthesize of regional livestock populations and in the types of management and feeding systems under which ruminant livestock are held, and increased demand of a wider range of quality attributes from animal agriculture, not just of the products themselves but also of the methods used in their production. The livestock sector will need to respond to newchallengesofincreasinglivestockproductivitywhileprotectingenvironmentand human health and conservingbiodiversity and natural resources. The micro-organisms in the digestive tracts of ruminant livestock have a profound in?uence on the conversion offeedinto end products, which can impact on the- imal and theenvironment. As the livestock sector grows particularly in developing countries, there will be an increasing need to understand these processes for b- ter management and use ofbothfeed and other natural resources that underpinthe development of sustainable feeding systems.

Editors and Affiliations

  • Animal Production and Health Section Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Vienna, Austria

    Harinder P.S. Makkar

  • CSIRO Livestock Industries, Queensland Bioscience Precinct, St Lucia, Australia

    Christopher S. McSweeney

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