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  • Book
  • © 2001

Proteoglycan Protocols

Editors:

  1. Renato V. Iozzo

    1. Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 171)

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Table of contents (51 protocols)

  1. Front Matter

    Pages i-xvi
    PDF

  2. Isolation and Purification

    1. Front Matter

      Pages 1-1
      PDF

    3. Isolation of Proteoglycans from Tendon

      • Kathryn G. Vogel, Julie A. Peters
      Pages 9-17

    7. Analysis of Proteoglycans and Glycosaminoglycans from Drosophila

      • William D. Staatz, Hidenao Toyoda, Akiko Kinoshita-Toyoda, Kimberlly Chhor, Scott B. Selleck
      Pages 41-52

    12. Particulate and Soluble Glycosaminoglycan-Synthesizing Enzymes

      • Geetha Sugumaran, Jeremiah E. Silbert
      Pages 103-116

    13. Disaccharide Composition of Hyaluronan and Chondroitin/Dermatan Sulfate

      • Anna H. K. Plaas, L. West, Ronald J. Midura, Vincent C. Hascall
      Pages 117-128

    17. Quantitation of Proteoglycans in Biological Fluids Using Alcian Blue

      • Madeleine Karlsson, Sven Björnsson
      Pages 159-173

    18. Cellulose Acetate Electrophoresis of Glycosaminoglycans

      • Yanusz Wegrowski, Francois-Xavier Maquart
      Pages 175-179
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About this book

Proteoglycans are some of the most elaborate macromolecules of mammalian and lower organisms. The covalent attachment of at least five types of glycosami- glycan side chains to more than forty individual protein cores makes these molecules quite complex and endows them with a multitude of biological functions. Proteoglycan Protocols offers a comprehensive and up-to-date collection of prepa- tive and analytical methods for the in-depth analysis of proteoglycans. Featuring st- by-step detailed protocols, this book will enable both novice and experienced researchers to isolate intact proteoglycans from tissues and cultured cells, to establish the composition of their carbohydrate moieties, to generate strategies for prokaryotic and eukaryotic expression, to utilize methods for the suppression of specific proteoglycan gene expression and for the detection of mutant cells and degradation products, and to study specific interactions between proteoglycans and extracellular matrix proteins as well as growth factors and their receptors. The readers will find concise, yet comprehensive techniques carefully drafted by leading experts in the field. Each chapter commences with a general Introduction, followed by a detailed Materials section, and an easy-to-follow Methods section. An asset of each chapter is the extensive notation that includes troubleshooting tips and practical considerations that are often lacking in formal methodology papers. The reader will find this section most valuable because it is clearly provided by experienced scientists who have first-hand knowledge of the techniques they outline. In addition, most of the chapters are well illustrated with examples of typical data generated with each method.
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Reviews

"The book is strategically subdivide into 3 sections (isolation and purification; expression, detection, and degradation; interactions) with a total of 51 chapters ( 19, 20, and 12 per subheading). Their contents dispel the clichés about work with sticky glycosaminoglycans being tedious, unrewarding, and mostly for the sake of old-fashioned...systematics....I expect even experts in certain areas to benefit from the whole book, whose rightful place will be on the bench, not on the shelf." - ChemBioChem

"This book will appeal to both those new to the subject and experts in the field as the protocols outlined are both wide ranging, comprehensive, and easy to follow....strongly recommend it to those new to proteoglycan research, and would regard it as an essential addition to any proteoglycan research laboratory library." - Molecular Biotechnology

"...this book will be a very useful tool for many specialists working in the fields of glycobiology, biotechnology, biochemistry, and experimental medicine. Detailed description of the methods employed for various aspects of proteoglycan analysis and comprehensive bibliography accompanying each chapter make this book especially valuable for a large audience of the mentioned specialists." - Biochemistry
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Editors and Affiliations

  • Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia

    Renato V. Iozzo

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Bibliographic Information

  • Book Title: Proteoglycan Protocols

  • Editors: Renato V. Iozzo

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592592090

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2001

  • Hardcover ISBN: 978-0-89603-759-5Published: 01 June 2001

  • Softcover ISBN: 978-1-61737-174-5Published: 10 November 2010

  • eBook ISBN: 978-1-59259-209-8Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 558

  • Topics: Biochemistry, general

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Buy it now

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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