Overview
- Editors:
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Heddwyn Jones
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University of Hertfordshire, Hatfield, UK
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Table of contents (37 protocols)
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Front Matter
Pages i-xiii
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Agrobacterium-Mediated Transformation
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- Patrick Gallois, Paulo Marinho
Pages 39-48
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- Jennifer F. Topping, Wenbin Wei, Michael C. Clarke, Paul Muskett, Keith Lindsey
Pages 63-76
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Direct Gene Transfer
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- Pilar Barcelo, Paul A. Lazzeri
Pages 113-123
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Use of Reporter Genes
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- Gillian A. Hull, Martine Devic
Pages 125-141
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- Michael R. Davey, Nigel W. Blackhall, J. Brian Power
Pages 143-148
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- Ian S. Curtis, J. Brian Power, Michael R. Davey
Pages 149-159
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Study of Gene Organization by Southern Blotting and Inverse PCR
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- Paolo A. Sabelli, Peter R. Shewry
Pages 161-180
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- Remko Offringa, Frederique van der Lee
Pages 181-195
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RNA Techniques for Studying Gene Expression
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- Paolo A. Sabelli, Peter R. Shewry
Pages 213-228
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- John Gatehouse, Andrew J. Thompson
Pages 229-238
About this book
The development of recombinant DNA technology and methods for transferring recombinant genes into plants has brought about significant advances in plant science. First, it has allowed investigation, using reporter genes, into the transcriptional regulation of plant genes—a key to the under standing of the biochemical basis of growth and development in plants. Second, gene transfer technology has facilitated the molecular cloning, by tagging genomic sequences, of important genes (e. g. , homeotic genes) whose gene products control the normal pattern of growth and differentia tion of plants. Third, overproducing foreign or endogenous proteins in plants can often lead to a better understanding of biochemical and physiological processes. Fourth, gene transfer technology has allowed the improvement of plant agricultural productivity. For example, plants have been engineered with improved viral resistance or the ability to withstand herbicide attack, therefore allowing a more effective use of herbicides to kill weeds. Fifth, there have been recent successes that demonstrate the potential use of plants as biotechnological chemical factories. For example, it is possible to use plants in the production of human antibodies and antigens of medical importance. It has been demonstrated recently that plants can be engineered to produce modified oils and even plastics! This paves the way to redirect agriculture from the production of surplus foods to the production of bio technological products of industrial importance.
Reviews
The chapters were written by outstanding scientists and [the book] can be recommended to any laboratory working with plants, providing tips and alternatives in case of problems. -Journal of Plant Physiology
Editors and Affiliations
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University of Hertfordshire, Hatfield, UK
Heddwyn Jones