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Protocols for Oligonucleotide Conjugates

Synthesis and Analytical Techniques

  • Book
  • © 1994

Overview

Authors:
  1. Sudhir Agrawal

    1. Worcester Foundation for Experimental Biology, Shrewsbury

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Part of the book series: Methods in Molecular Biology (MIMB, volume 26)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Protecting Groups in Oligonucleotide Synthesis

    • Etienne Sonveaux
    Pages 1-71

  3. Incorporation of Modified Bases into Oligonucleotides

    • Rich B. Meyer Jr
    Pages 73-91

  4. Functionalization of Oligonucleotides with Amino Groups and Attachment of Amino Specific Reporter Groups

    • Sudhir Agrawal
    Pages 93-120

  5. Functionalization of Oligonucleotides by the Incorporation of Thio-Specific Reporter Groups

    • Jacqueline A Fidanza, Hiroaki Ozaki, Larry W. McLaughlin
    Pages 121-143

  6. Postsynthesis Functionalization of Oligonucleotides

    • Barbara C. E Chu, Leslie E. Orgel
    Pages 145-165

  7. Oligonucleotide-Enzyme Conjugates

    • Jerry L Ruth
    Pages 167-185

  8. Oligonucleotides Containing Degenerate Bases

    • Paul Kong Thoo Lin, Daniel M. Brown
    Pages 187-206

  9. Synthesis of [15N]-Labeled DNA Fragments

    • Roger A Jones
    Pages 207-231

  10. Analysis and Purification of Synthetic Oligonucleotides by High-Performance Liquid Chromatography

    • William J. Warren, George Vella
    Pages 233-264

  11. Sequence Analysis of Oligodeoxyribonucleotides

    • Diane M. Black, Peter T. Gilham
    Pages 265-276

  12. Gel-Capillary Electrophoresis Analysis of Oligonucleotides

    • Alex Andrus
    Pages 277-300

  13. Nuclear Magnetic Resonance Studies of Oligonucleotides

    • Jerzy W. Jaroszewski, Siddhartha Roy, Jack S. Cohen
    Pages 301-317

  14. Mass Spectrometry of Nucleotides and Oligonucleotides

    • Thomas D. McClure, Karl H. Schram
    Pages 319-345

  15. Extracting Thermodynamic Data From Equilibrium Melting Curves for Oligonucleotide Order-Disorder Transitions

    • Kenneth J. Breslauer
    Pages 347-372

  16. Back Matter

    Pages 373-377
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Reviews

...an extremely useful and well-produced volume...a very well-qualified and prestigious group of authors...produce an up-to-date review of current topics in this important and growing field. - Trends in Biotechnology

Authors and Affiliations

  • Worcester Foundation for Experimental Biology, Shrewsbury

    Sudhir Agrawal

Bibliographic Information

  • Book Title: Protocols for Oligonucleotide Conjugates

  • Book Subtitle: Synthesis and Analytical Techniques

  • Authors: Sudhir Agrawal

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59259-513-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1994

  • Softcover ISBN: 978-0-89603-252-1Published: 30 November 1993

  • eBook ISBN: 978-1-59259-513-6Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 377

  • Topics: Organic Chemistry

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