Skip to main content
SpringerLink
Log in

Identification of estrone in pomegranate (Punica granatum) extracts by liquid chromatography-tandem mass spectrometry

  • Research Article
  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

There were some reports that the pomegranate juice, seeds, and peel extracts contain steroid hormones including estrone, but the results were controversial. This study was performed to identify estrone in pomegranate extracts through a more sensitive and validated technology. Separation and determination of estrone was carried out using high performance liquid chromatography (HPLC) system with triple quadrupole mass spectrometry. Low concentration of estrone (4.5±0.38 ng/mL) was detected in pomegranate extracts with adequate precision value. The amount of estrone in pomegranate extracts was calculated as 16.4, 18.2, and 20.2 ng/mL, respectively, adjusted by extraction efficiency (average 25.6%) in the precision verification.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Longtin R. The pomegranate: Nature’s power fruit. J. Natl. Cancer I. 95: 346–348 (2003)

    Article  Google Scholar 

  2. Schubert SY, Lansky EP, Neeman I. Antioxidant and eicosanoid enzyme inhibition properties of pomegranate seed oil and fermented juice flavonoids. J. Ethnopharmacol. 66: 11–17 (1999)

    Article  CAS  Google Scholar 

  3. Anesini C, Perez C. Screening of plants used in Argentine folk medicine for antimicrobial activity. J. Ethnopharmacol. 39: 119–128 (1993)

    Article  CAS  Google Scholar 

  4. Braga LC, Leite AA. Xavier KG. Synergistic interaction between pomegranate extract and antibiotics against Staphylococcus aureus. Can. J. Microbiol. 51: 541–547 (2005)

    Article  CAS  Google Scholar 

  5. Zhanag J, Zhan B, Yao X, Gao Y, Shong J. Antiviral activity of tannin from the pericarp of Punica granatum L. against genital Herpes virus in vitro. Zhongguo Zhong Yao Za Zhi 20: 556–558 (1995)

    Google Scholar 

  6. Jeune MA, Kumi-Diaka J, Brown J. Anticancer activities of pomegranate extracts and genistein in human breast cancer cells. J. Med. Food 8: 469–475 (2005)

    Article  CAS  Google Scholar 

  7. Kim ND, Mehta B, Yu W, Neeman I, Livney T, Amichay A, Poirier D, Nicholls P, Kirby A, Jiang W, Mansel R, Ramachandran C, Rabi T, Kaplan B, Lansky E. Chemopreventive and adjuvant therapeutic potential of pomegranate (Punica granatum) for human breast cancer. Breast Cancer Res. Tr. 71: 203–217 (2002)

    Article  CAS  Google Scholar 

  8. Espin JS, Garcia-Conesa MT, Tomas-Barberan FA. Nutraceuticals: Facts and fiction. Phytochemistry 68: 2986–3008 (2007)

    Article  CAS  Google Scholar 

  9. Gil MI, García-Viguera C, Artés F, Tomás-Barberán FA. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. J Sci. Food Agr. 68: 77–81 (1995)

    Article  Google Scholar 

  10. Lee JH, Kim SD, Lee JY, Kim KN, Kim HS. Analysis of flavonoids in concentrated pomegranate extracts by HPLC with diode array detection. Food Sci. Biotechnol. 14: 171–174 (2005)

    CAS  Google Scholar 

  11. Melgarejo P, Artes F. Total lipid content and fatty acid composition of oilseed from lesser known sweet pomegranate clones. J. Sci. Food Agr. 80: 1452–1454 (2000)

    Article  CAS  Google Scholar 

  12. Hopkins CY, Chrisholm MJ. A survey of the conjugated fatty acids of seed oils. J. Am. Oil Chem. Soc. 45: 176–182 (1968)

    Article  CAS  Google Scholar 

  13. Heftmann E, Ko ST, Bennet RD. Identification of estrone in pomegranate seeds. Phytochemistry 5: 1337–1340 (1966)

    Article  CAS  Google Scholar 

  14. Choi DW, Kim JY, Choi SH, Jung HS, Kim HJ, Cho HJ, Cho SY, Kang CS, Chang SY. Identification of steroid hormones in pomegranate (Punica granatum) using HPLC and GC-mass spectrometry. Food Chem. 96: 562–571 (2006)

    Article  CAS  Google Scholar 

  15. Tong P, Kasuga Y, Khoo CS. Liquid chromatographic-mass spectrometric method for detection of estrogen in commercial oils and in fruit seed oils. J. Food Compos. Anal. 19: 150–156 (2006)

    Article  CAS  Google Scholar 

  16. Elswijk DA, Schobel UP, Lansky EP, Irth H, Greef J. Rapid dereplication of estrogenic compounds in pomegranate (Punica granatum) using on-line biochemical detection coupled to mass spectrometry. Phytochemistry 65: 233–241 (2004)

    Article  Google Scholar 

  17. Farré M, Kuster M, Brix R, Rubio F, Alda ML, Barceló D. Comparative study of an estradiol enzyme-linked immunosorbent assay kit, liquid chromatography-tandem mass spectrometry, and ultra performance liquid chromatography-quadrupole time of flight mass spectrometry for part-per-trillion analysis of estrogens in water samples. J. Chromatogr. A 1160: 166–175 (2007)

    Article  Google Scholar 

  18. Hosogia J, Tanaka H, Fujita K, Kuwabara T, Ikegawa S, Kobayashi N, Mano N, Goto J. LC-MS/MS coupled with immunoaffinity extraction for determination of estrone, 17β-estradiol, and estrone 3-sulfate in human plasma. J. Chromatogr. B 878: 222–227 (2010)

    Article  Google Scholar 

  19. US EPA. Revised Assessment of Detection and Quantitation Approaches. EPA-821-B-04-005. Office of Water, US Environmental Protection Agency, Washington, DC, USA (2004)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Natural Food Sciences, Eulji University, Seongnam, Gyeonggi, 461-713, Korea

    Jung Hoan Kim

  2. School of Human and Environmental Sciences, Eulji University, Seongnam, Gyeonggi, 461-713, Korea

    Young Lim Kho

  3. Department of Emergency Medicine, East-West Neo Medical Center, Kyung Hee University, Seoul, 134-727, Korea

    Woong Jung

  4. Research Center, Hanil PFC Co., Ltd., Anseong, Gyeonggi, 456-825, Korea

    Dohyeong Kwon

Authors
  1. Young Lim Kho
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Woong Jung
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dohyeong Kwon
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jung Hoan Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jung Hoan Kim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kho, Y.L., Jung, W., Kwon, D. et al. Identification of estrone in pomegranate (Punica granatum) extracts by liquid chromatography-tandem mass spectrometry. Food Sci Biotechnol 19, 809–813 (2010). https://doi.org/10.1007/s10068-010-0113-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-010-0113-z

Keywords

Search

Navigation