Abstract
Out of 12 nontuberculous mycobacteria (NTM) species, 5 were identified as Mycobacterium tuberculosis (MTB) by GeneXpert at a bacterial load of 106. Notably, two species, including M. abscessus and M. smegmatis, were flagged as RIF-resistant MTB due to the high C(t) value of Probe E. In conclusion, our data have demonstrated that the misdiagnosis of MTB by GeneXpert assay is observed in five NTM species at a high bacterial load.
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Acknowledgments
This work was supported by the National Key Research Program of China (2014ZX10003002) and the National Natural Science Foundation of China (81401739). We would like to thank members of the National Tuberculosis Reference Laboratory at the Chinese Center for Disease Control and Prevention for their technical assistance.
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This work was supported by the Major State Basic Research Development Program of China (2014CB744403) and the National Natural Science Foundation of China (81401739).
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The authors declare that they have no competing interests.
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This study was approved by the Ethic Committee of Chinese Center for Disease Control and Prevention.
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Pang, Y., Lu, J., Su, B. et al. Misdiagnosis of tuberculosis associated with some species of nontuberculous mycobacteria by GeneXpert MTB/RIF assay. Infection 45, 677–681 (2017). https://doi.org/10.1007/s15010-017-1044-x
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DOI: https://doi.org/10.1007/s15010-017-1044-x