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Transcriptional profiling in cotton associated with Bacillus subtilis (UFLA285) induced biotic-stress tolerance

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Abstract

Plant growth promoting rhizobacteria (PGPR) confer disease resistance in many agricultural crops. In the case of Bacillus subtilis (UFLA285) isolated from the cotton producing state of Mato Grosso (Brazil), in addition to inducing foliar and root growth, disease resistance against damping-off caused by Rhizoctonia solani was observed. The aim of this cotton study was to identify gene transcriptional events altered with exposure to the PGPR strain UFLA285 in infected plants. Global gene transcription was profiled using a commercially-available cotton gene chip; cotton plants with and without UFLA285-seed treatment were infected with R. solani 9-days after planting and harvested on day14. Microarray data of stem tissue revealed 247 genes differentially regulated in infected plants, seed treated versus untreated with UFLA285. Transcripts encoding disease resistance proteins via jasmonate/ethylene signaling as well as osmotic regulation via proline synthesis genes were differentially expressed with UFLA285 induction. Consistent with transcriptional regulation, UFLA285 increased plant-proline accumulation and dry weight. This study has identified transcriptional changes in cotton, induced by the beneficial soil bacterium UFLA285 and associated with disease control.

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Acknowledgements

Financial assistance was provided in part by a grant from the Robert Welch Foundation (D-1478) and comments on an earlier draft of this manuscript were provided by Dr. Huazhong Shi.

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Authors and Affiliations

  1. Departamento de Fitopatologia, Universidade Federal de Lavras, CP 3037, CEP 37200–000, Lavras, MG, Brazil

    Flavio H. V. Medeiros, Ricardo M. Souza, Fernanda C. L. Medeiros & Henrique M. Ferro

  2. Departments of Chemistry and Biology, Texas Tech University, Lubbock, TX, 79409, USA

    Huiming Zhang & Paul W. Paré

  3. TAES, Rt. 3, Box 219, Lubbock, TX, 79403, USA

    Terry Wheeler

  4. United States Department of Agriculture, 3810 4th St, Lubbock, TX, 79415, USA

    Paxton Payton

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  1. Flavio H. V. Medeiros
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  2. Ricardo M. Souza
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  3. Fernanda C. L. Medeiros
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  4. Huiming Zhang
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  5. Terry Wheeler
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  7. Henrique M. Ferro
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  8. Paul W. Paré
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Correspondence to Paul W. Paré.

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Responsible Editor: Jesus Mercado-Blanco.

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Medeiros, F.H.V., Souza, R.M., Medeiros, F.C.L. et al. Transcriptional profiling in cotton associated with Bacillus subtilis (UFLA285) induced biotic-stress tolerance. Plant Soil 347, 327–337 (2011). https://doi.org/10.1007/s11104-011-0852-5

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  • DOI: https://doi.org/10.1007/s11104-011-0852-5

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