Abstract
Contemporary microarrayers of contact or non-contact format used in protein microarray fabrication still suffer from a number of problems, e.g. generation of satellite spots, inhomogeneous spots, misplaced or even absent spots, and sample carryover. In this paper, a new concept of non-contact sample deposition that reduces such problems is introduced. To show the potential and robustness of this pressure-assisted deposition technique, different sample solutions known to cause severe problems or to be even impossible to print with conventional microarrayers were accurately printed. The samples included 200 mg mL–1 human serum albumin, highly concentrated sticky cell adhesion proteins, pure high-salt cell-lysis buffer, pure DMSO, and a suspension of 5-μm polystyrene beads. Additionally, a water-immiscible liquid fluorocarbon, which was shown not to affect the functionality of the capture molecules, was employed as a lid to reduce evaporation during microarray printing. The fluorocarbon liquid lid was shown to circumvent hydrolysis of water-sensitive activated surfaces during long-term deposition procedures.
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Acknowledgements
We thank Sylvia Adam and Heiko Steuer (both NMI) for performing the cell adhesion assays. This work was funded by the European Union as part of the program “Nanobiotechnology with Self-Organising Structures (NABIS)”, contract no. NMP4-CT-2003–505311. Financial support from the Swedish Research Council is also gratefully acknowledged.
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Hartmann, M., Sjödahl, J., Stjernström, M. et al. Non-contact protein microarray fabrication using a procedure based on liquid bridge formation. Anal Bioanal Chem 393, 591–598 (2009). https://doi.org/10.1007/s00216-008-2509-7
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DOI: https://doi.org/10.1007/s00216-008-2509-7