1. Rapid transfer of low copy-number episomal plasmids fromSaccharomyces cerevisiae to Escherichia coli by electroporation

   • Laura Gunn
   • Jac A. Nickoloff

   Research Pages: 79 - 84

2. Rapid identification of polymorphic CA-repeats in YAC clones

   • Galit Rotman
   • Lina Vanagaite
   • Yosef Shiloh

   Research Pages: 85 - 92

3. PCR-mediated recombination and mutagenesis

   • Robert M. Horton

   Protocol Pages: 93 - 99

4. Xenopus oocytes as a heterologous expression system for plant proteins

   • Frederica L. Theodoulou
   • Anthony J. Miller

   Protocol Pages: 101 - 115

5. Irradiation and fusion gene transfer

   • Michael A. Walter
   • Peter N. Goodfellow

   Protocol Pages: 117 - 128

6. Selection of primers for polymerase chain reaction

   • Wojciech Rychlik

   Protocol Pages: 129 - 134

7. Purification and cloning of DNA fragments fractionated on agarose gels

   • Hugh G. Griffin
   • Michael J. Gasson

   Protocol Pages: 135 - 138

8. The biotechnology and applications of antibody engineering

   • Ralph Rapley

   Review Pages: 139 - 154

9. RNA viruses as vectors for the expression of heterologous proteins

   • Sondra Schlesinger

   Review Pages: 155 - 165

10. Increased reliability of selective PCR by using additionally mutated primers and a commercialTaq DNA polymerase enhancer

    • Angelo De Milito
    • Marinunzia Catucci
    • Pier Egisto Valensin

    Departments Pages: 166 - 169

11. European bioinformatics institute (EBI)

    • Hugh Griffin

    Departments Pages: 170 - 171

12. Perspectives in protein engineering and complementary technologies, Oxford, UK, September 13–17, 1994

    • Dan Tawfik
    • Ralph Rapley

    Departments Pages: 172 - 173

13. Software update

    Departments Pages: 174 - 174

14. From genetics to gene therapy

    • L. Stefani

    Book review Pages: 175 - 175

15. New products for molecular biology

    Departments Pages: 176 - 179

16. Calendar

    Departments Pages: 180 - 180