Summary
For the first time, DNA sequence data were obtained for three species of Trichostrongylus from Russia. Internal transcribed spacer (ITS-2) of ribosomal DNA was sequenced for T. axei, T. colubriformis and T. probolurus from sheep from the Moscow region. ITS-2 rDNA length was estimated as 238 nucleotides for T. colubriformis and T. probolurus and 237 nucleotides for T. axei. The G+C content of the ITS-2 sequences of T. colubriformis, T. axei and T. probolurus were 31 %, 32 % and 34 % respectively. The level of interspecific differences in ITS-2 of rDNA of T. axei, T. probolurus and T. colubriformis ranged from 3 to 4 %. The ITS-2 sequences from the Russian specimens were compared with those of T. axei, T. probolurus and T. colubriformis from Australia and Germany. Intraspecific variation ranged from 0 % in T. colubriformis to 3.0 % in T. axei.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Kkbaev, M. Sh., Vodyanov, A. A., Kosminkov, N. E., Yatusevitch, A. I., Pashkin, P. I., Vasylevitch, F. I. (1998): Parasitology and invasive diseases of animals. Pub. House Kolos, Moscow
Asadov, S. M. (1960): Fauna of helminthes of ruminants of USSR and analysis of its distribution and ecology. Pub. House of Azerbaijan SSR Academy of Sciences, Baku
Boev, S. N., Sokolova, I. B., Panin, V. Ya. (1963): Helminthes of ruminants of Kazakhstan. V. II. Pub. House of Kasakh SSR Academy of Sciences, Alma-Ata
Chilton, N. B., Hoste, H., Newton, L. A., Beveridge, I., Gasser, R. B. (1998): Common secondary structures for the second internal transcribed spacer pre-rRNA of two subfamilies of trichostrongylid nematodes. Int. J. Parasitol., 28: 1765–1773
Dallas, J. F., Irvine, R. J., Halvorsen, O. (2000): DNA evidence that Ostertagia gruehneri and Ostertagia arctica (Nematoda: Ostertagiinae) in reindeer from Norway and Svalbard are conspecific. Int. J. Parasitol., 30: 655–658
Gasser, R. B., Chilton, N. B., Hoste, H., Beveridge, I. (1993): Rapid sequencing of rDNA from single worms and eggs of parasitic helminths. Nucleic Acids Res., 21: 2525–2526
Govorka, Ya., Maklakova, L. P., Mitukh, Ya., Pelgunov, A. N., Rykovskyi, A. S., Semenova, M. K., Sonin, M. D., Erkhardova-Kotrla, B., Yurashek, V. (1988): Helminthes of wild ungulates in Eastern Europe. Pub. House Nauka, Moscow
Heise, M., Epe, C., Schneider, T. (1999): Differences in the second internal transcribed spacer (ITS-2) of eight species of gastrointestinal nematodes of ruminants. J. Parasitol., 85: 431–435
Hoste, H., Gasser, R. B., Chilton, N. B., Mallet, S., Beveridge, I. (1993): Lack of intraspecific variation in the second internal transcribed spacer (ITS-2) of Trichostrongylus colubriformis rDNA. Int. J. Parasitol., 23: 1069–1071
Hoste, H., Chilton, N. B., Gasser, R. B., Beveridge, I. (1995): Differences in the second internal transcribed spacer (ribosomal DNA) between five species of Trichostrongylus (Nematoda: Trichostrongylidae). Int. J. Parasitol., 25: 75–80
Hoste, H., Chilton, N. B., Beveridge, I., Gasser, R. B. (1998): A comparison of the first internal transcribed spacer of ribosomal DNA in seven species of Trichostrongylus (Nematoda: Trichostrongylidae). Int. J. Parasitol., 28: 1251–1260
Ivashkin, V. M., Oripov, A. O., Sonin, M. D. (1989): Keys to helminthes of small cattle. Pub. House Nauka, Moscow
McMurtry, L. W., Donaghy, M. J., Vlassoff, A., Douch, P. G. C. (2000): Distinguishing morphological features of the third larval stage of ovine Trichostrongylus spp. Vet. Parasitol., 90: 73–81
Pryadko, E. I. (1976): Helminthes of deer. Pub. House Nauka of Kazakh SSR, Alma-Ata
Skrjabin, K. I., Shikhobalova, N. P., Shults R. S. (1954): Essentials of nematodology III. Trichostrongyloids of animals and man. Pub. House of Academy of Sciences USSR, Moscow
Skrjabin, K. I., Petrov, A. M. (1964): Essentials of veterinary nematodology. Pub. House Kolos, Moscow
Swofford, D. L. (1998): PAUP*. Phylogenetic analysis using parsimony. Version 4. Sinauer, Sunderland, MA
Wimmer, B., Craig, B. H., Pilkington, J. G., Pemberton, J. M. (2004): Non-invasive assessment of parasitic nematode species diversity in wild Soay sheep using molecular markers. Int. J. Parasitol., 34: 625–631
Author information
Authors and Affiliations
About this article
Cite this article
Kuznetsov, D.N., Kuznetsova, N.A. Sequences of the second internal transcribed spacer of ribosomal DNA for three species of Trichostrongylus (Nematoda: Trichostrongylidae) from sheep in Russia. Helminthologia 44, 43–46 (2007). https://doi.org/10.2478/s11687-007-0002-z
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.2478/s11687-007-0002-z