Skip to main content
SpringerLink
Log in

Proteomic analysis of differentially expressed serum proteins in lung cancer

  • Research Article
  • Published:
Central European Journal of Biology

Abstract

Lung cancer continues to represent a major public health concern with high morbidity and mortality worldwide. Early detection of lung cancer is problematic due to a lack of diagnostic markers with high sensitivity and specificity. To determine the differently expressed proteins in the serum of lung cancer and identify the function of such proteins, two-dimensional electrophoresis (2DE) and liquid chromatography mass spectrometry (LC-MS) were used to screen the serum of lung cancer model induced by 4-(methylnitrosoamino)-1-(3-pyridyl)-1-butanone (NNK). A total of 25 protein spots were qualitatively different and 6 were quantitatively different in the serum from rats bearing induced lung cancer when compared with normal controls. Two of the proteins that showed major changes in concentration in sera were identified to be Immunoglobulin γ 2A chain C region (heavy chain) and Transferrin by LC-MS/MS.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Commonly Diagnosed Cancers Worldwide, Cancer Research UK, 2005

  2. Jemal A., Tiwari R.C., Murray T., Ward E., Samuels A., Ghafoor A., et al., Cancer statistics, CA Cancer J. Clin., 2005, 54, 8–29

    Article  Google Scholar 

  3. Li M., Ye B., Zhang Y., Chen H., Xia D., Liu M., et al., Proteomic analysis of serum in lung cancer induced by 3-methylcholanthrene, J. Biomed. Biotechnol., 2009, 1–12

  4. Pandey A., Mann M., Proteomics to study genes and genomes, Nature, 2000, 405, 837–846

    Article  PubMed  CAS  Google Scholar 

  5. Alessandro R., Fontana S., Kohn E., De Leo G., Proteomic strategies and their application in cancer research, Tumori., 2005, 91, 447–455

    PubMed  CAS  Google Scholar 

  6. Koomen J.M., Haura E.B., Bepler G., Sutphen R., Remily-Wood E.R., Benson K., et al., Proteomic contributions to personalized cancer care, Mol. Cell. Proteomics, 2008, 7, 1780–1794

    Article  PubMed  CAS  Google Scholar 

  7. Peng X.Q., Wang F., Geng X., Current advances in tumor proteomics and candidate biomarkers for hepatic cancer, Expert Rev. Proteomics, 2009, 6, 551–561

    Article  PubMed  CAS  Google Scholar 

  8. Simpson R.J., Bernhard O.K., Greening D.W., Moritz R.L., Proteomics-driven cancer biomarker discovery: looking to the future, Curr. Opin. Chem. Biol., 2008, 12, 72–77

    Article  PubMed  CAS  Google Scholar 

  9. Cho H.J., Baek K.E., Park S.M., Kim K.I., Choi Y.L., Cho H.J., et al., RhoGDI2 expression is associated with tumor growth and malignant progression of gastric cancer, Clin. Cancer Res., 2009, 15, 2612–2619

    Article  PubMed  CAS  Google Scholar 

  10. Hongsachart P., Huang-Liu R., Sinchaikul S., Pan F.M., Phutrakul S., Chuang Y.M., et al., Glycoproteomic analysis of WGA-bound glycoprotein biomarkers in sera from patients with lung adenocarcinoma, Electrophoresis, 2009, 30, 1206–1220

    Article  PubMed  CAS  Google Scholar 

  11. Ono M., Matsubara J., Honda K., Sakuma T., Hashiguchi T., Nose H., et al., Prolyl 4-hydroxylation of alpha-fibrinogen: A novel protein modification revealed by plasma proteomic, J. Biol. Chem., 2009, 284, 29041–29049

    Article  PubMed  CAS  Google Scholar 

  12. Schulz D.M., Böllner C., Thomas G., Atkinson M., Esposito I., Hofler H., et al., Identification of differentially expressed proteins in triplenegative breast carcinomas using DIGE and mass spectrometry, J. Proteome Res., 2009, 8, 3430–3438

    Article  PubMed  CAS  Google Scholar 

  13. Vellaichamy A., Sreekumar A., Strahler J.R., Rajendiran T., Yu J., Varambally S., et al., Proteomic interrogation of androgen action in prostate cancer cells reveals roles of aminoacyl tRNA synthetases, PLoS One, 2009, 4, e7075

    Article  PubMed  Google Scholar 

  14. Mathivanan S., Lim J.W., Tauro B.J., Ji H., Moritz R.L., Simpson R.J., Proteomic analysis of A33-immunoaffinity-purified exosomes released from the human colon tumor cell line LIM1215 reveals a tissue-specific protein signature, Mol. Cell. Proteomics, 2010 9, 197–208

    Article  PubMed  CAS  Google Scholar 

  15. Sagynaliev E., Steinert R., Nestler G., Lippert H., Knoch M., Reymond M.A., Web-based datawarehouse on gene expression in human colorectal cancer, Proteomics, 2005, 5, 3066–3078

    Article  PubMed  CAS  Google Scholar 

  16. Sung H.J., Cho J.Y., Biomarkers for the lung cancer diagnosis and their advances in proteomics, BMB reports, 2008, 41, 615–625

    Article  PubMed  CAS  Google Scholar 

  17. Zhang Y., Li M., Liu Y., Han N., Zhang K., Xiao T., et al., ENO1 protein levels in the tumor tissues and circulating plasma samples of non-small cell lung cancer patients, Chinese Journal of lung cancer, 2010, 13, 1089–1093

    PubMed  CAS  Google Scholar 

  18. Ugurel S., Bell N., Sucker A., Zimpfer A., Rittgen, W., Schadendorf D., Tumor type M2 pyruvate kinase (TuM2-PK) as a novel plasma tumor marker in melanoma, Int. J. Cancer, 2005, 117,5, 825–830

    Article  PubMed  CAS  Google Scholar 

  19. Belinsky A., White M., Boucheron A., Richardson C., Swenberg A., Anderson M., Accumulation and persistence of DNA adducts in respiratory tissue of rats following multiple administrations of the tobacco specific carcinogen 4-(N-Methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone, Cancer Res., 1986, 46, 1280–1284

    PubMed  CAS  Google Scholar 

  20. Tuck M.K., Chan D.W., Chia D., Godwin A.K., Grizzle W.E., Krueger K.E., et al., Standard operating procedures for serum and plasma collection: early detection research network consensus statement Standard Operating Procedure Integration Working Group, J. Proteome Res., 2009, 8, 113–117

    Article  PubMed  CAS  Google Scholar 

  21. Anderson N.L., Anderson N.G., The human plasma proteome: history, character, and diagnosis prospects, Mol. Cell. Proteomics, 2002, 1, 845–67

    Article  PubMed  CAS  Google Scholar 

  22. Laemmli U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 1970, 227, 680–685

    Article  PubMed  CAS  Google Scholar 

  23. Bradford M.M., Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 1976, 72, 248–254

    Article  PubMed  CAS  Google Scholar 

  24. Huebers H.A., Finch C.A., The physiology of transferrin and transferrin receptors, Physiol. Rev., 1987, 67, 520–582

    PubMed  CAS  Google Scholar 

  25. Kuvibidila S., Gauthier T., Warrier R.P., Rayford W., Increased levels of serum transferrin receptor and serum transferrin receptor/log ferritin ratios in men with prostate cancer and the implications for body-iron stores, J. Lab. Clin. Med., 2004, 144, 176–182

    Article  PubMed  CAS  Google Scholar 

  26. Faulk W.E., His B.L., Stevens P.J., Transferrin and transferrin receptors in carcinoma of the breast, Lancet, 1980, 2, 390–392

    Article  PubMed  CAS  Google Scholar 

  27. Lamoureux G., Mandeville R., Poisson R., Poisson S.L., Jolicoeur R., Biological markers and breast cancer. A multiparametric study, increased serum protein levels, Cancer, 1982, 49, 502–512

    Article  PubMed  CAS  Google Scholar 

  28. Stevens G., Graubard I., Micozzi S., Neriishi K., Blumberg B.S., Moderate elevation of body iron level and increase risk of cancer occurrence and death, Int. J. Cancer, 1994, 56, 364–369

    Article  PubMed  CAS  Google Scholar 

  29. Kassie F., Anderson L.B., Scerber R., Yu N., Lahti D., Upadhyaya P., Indole-3-carbinol Inhibits 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone Plus Benzo(a)pyrene-Induced Lung Tumorigenesis in A/J Mice and Modulates Carcinogen-Induced Alterations in Protein Levels, Cancer Res., 2007, 67, 6502–6511

    Article  PubMed  CAS  Google Scholar 

  30. Knekt P.M., Reunanen A., Takkunen H., Body iron stores and risk of cancer, Int. J. Cancer, 1994, 56, 379–82

    Article  PubMed  CAS  Google Scholar 

  31. Eberini I., Miller I., Zancan V., Bolego C., Puglisi L., Gemeiner M., et al., Proteins of rat serum IV. Timecourse of acute-phase protein expression and its modulation by indomethacine, Electrophoresis, 1999, 20, 846–853

    Article  PubMed  CAS  Google Scholar 

  32. Eberini I., Agnello D., Miller I., Villa P., Fratelli M., Ghezzi P., et al., Proteins of rat serum V: adjuvant arthritis and its modulation by nonsteroidal antiinflammatory drugs, Electrophoresis, 2000, 21, 2170–2179

    Article  Google Scholar 

  33. Haynes P., Miller I., Aebersold R., Gemeiner M., Eberini I., Lovati M.R., et al., Proteins of rat serum: I establishing a reference two-dimensional electrophoresis map by immunodetection and microbore high performance liquid chromatography electrospray mass spectrometry, Electrophoresis, 1998, 19, 1484–1492

    Article  PubMed  CAS  Google Scholar 

  34. Kimoto Y., Expression of heavy-chain constant region of immunoglobulin and T-cell receptor gene transcripts in human non-hematopoietic tumor cell lines, Genes Chromosomes Cancer, 1998, 22, 83–86

    Article  PubMed  CAS  Google Scholar 

  35. Qiu X., Zhu X., Zhang L., Mao Y., Zhang J., Hao P., et al., Human epithelial cancers secrete immunoglobulin G with unidentified specificity to promote growth and survival of tumor cells, Cancer Research, 2003, 63, 6488–6495

    PubMed  CAS  Google Scholar 

  36. Olubuyide I.O., Salimonu L.S., Adeniran S.O., Soluble immune complexes and immunoglobulin (IgG, IgA and IgM) levels in Nigerians with primary liver cell carcinoma, Afr. J. Med. Sci., 1993, 22, 57–62

    CAS  Google Scholar 

  37. Ouyang G., Chen Y., Ruan L., Differential phosphoproteome analysis of nasopharyngeal carcinoma and normal nasopharyngeal epithelial tissues, Int. J. Path. Clin. Med., 2007, 27, 461–469

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, Hamdard University, 110062, New Delhi, India

    Shilpa Bhatnagar, Naveen Chaudhary, Shabnam Malik & Swatantra K. Jain

  2. Amity Institute of Pharmacy, Amity University, 201303, Noida, Uttar Pradesh, India

    Deepshikha Pande Katare

Authors
  1. Shilpa Bhatnagar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Naveen Chaudhary
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shabnam Malik
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Deepshikha Pande Katare
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Swatantra K. Jain
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Swatantra K. Jain.

About this article

Cite this article

Bhatnagar, S., Chaudhary, N., Malik, S. et al. Proteomic analysis of differentially expressed serum proteins in lung cancer. cent.eur.j.biol. 7, 343–353 (2012). https://doi.org/10.2478/s11535-012-0016-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2478/s11535-012-0016-5

Keywords

Search

Navigation