Abstract
The so-called ion-step method represents a newly developed measurement concept for potentiometric detection and quantification of adsorbed biomolecules in which modified ion-sensitive field-effect transistors (ISFETs) are used. The ion-step method is based on a dynamic measuring principle, whereas many other potentiometric methods are static and measure in a state of thermo-dynamic equilibrium. A number of authors report on measuring protein adsorption by equilibrium potentiometry, but in all measurements the observed responses were poor (1, 2). The new measuring method is therefore an important alternative method for detection of adsorbed protein. In Subheading 1.2., a short explanation will be given for the disappointing results obtained with detection of protein by equilibrium potentiometry. Thereafter, the ion-step method will be described briefly and at the end of this introduction, the use of an ISFET affinity sensor for the measurement of heparin will be described.
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Aizawa, M (1978) Electrochemical determination of IgG with an antibody bound membrane. J. Membr. Sci 4, 221–228.
Yamamoto, N., Nagasawa, Y., Sawai, Y., Suda, M., and Tsubomura, T. H. (1978) Potentiometric investigations of antigen-antibody and enzyme-enzyme inhibitor reactions using chemically modified metal electrodes J Immunol. Meth 22, 309–317.
Schasfoort, R. B. M, Bergveld, P., Kooyman, R P. H., and Greve, J. (1990) Possibilities and limitations of direct detection of protein charges by means of an immunological field-effect transistor. Anal. Chim Acta 238, 323–329.
Eijkel, J. C. T. (1995) Potentiometric detection and characterization of adsorbed protein using stimulus-response measurement techniques. PhD thesis, University of Twente, Enschede, The Netherlands, ISBN 90-9008615-3
Schasfoort, R. B. M., Kooyman, R. P. H., Bergveld, P., and Greve, J. (1990) A new approach to ImmunoFET operation. Biosens Bioelectron. 5, 103–124.
Van Kerkhof, J. C, Eijkel, J. C. T., and Bergveld, P. (1994) ISFET responses on a stepwise change in electrolyte concentration at constant pH. Sensors Actuators B 18, 56–59.
Van Kerkhof, J. C, Bergveld, P., and Schasfoort, R. B M. (1995) The ISFET based heparin sensor with a monolayer of protamine as affinity ligand. Biosens. Bioelectron. 10, 269–282.
Van Kerkhof, J. C, Bergveld, P., and Schasfoort, R. B. M. (1993) Development of an ISFET based heparin sensor using the ion-step measuring method. Biosens Bioelectron 8, 463–472
Schasfoort, R. B. M., Keldermans, C. E. J. M., Kooyman, R. P. H., Bergveld, P., and Greve, J. (1990) Competitive immunological detection of progesterone by means of the ion-step induced response of an ImmunoFET. Sensors Actuators B1, 368–372.
Van Kerkhof, J. C. (1994) The development of an ISFET-based heparin sensor. PhD thesis, University of Twente, Enschede, The Netherlands, ISBN 90-9007514-3, Chapter 7.
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© 1998 Humana Press Inc., Totowa, NJ
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Besselink, G.A.L., Bergveld, P. (1998). ISFET affinity sensor. In: Rogers, K.R., Mulchandani, A. (eds) Affinity Biosensors. Methods in Biotechnology, vol 7. Humana Press. https://doi.org/10.1385/0-89603-539-5:173
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DOI: https://doi.org/10.1385/0-89603-539-5:173
Publisher Name: Humana Press
Print ISBN: 978-0-89603-539-3
Online ISBN: 978-1-59259-485-6
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